Metabolism

Question 1

Name the 8 enzymes used in the TCA cycle in order

Answer 1

1. Citrate synthase
2. Aconitase
3. Isocitrate dehydrogenase
4. a-Ketoglutanate dehydrogenase
5. Succinyl-CoA synthetase
6. Succinate dehydrogenase
7. Fumerase
8. Malate dehydrogenase

Question 2

Between which 2 intermediates in the TCA cycle is GTP formed from GDP and Pi?

Answer 2

Succinyl CoA and Succinate

Question 3

Between which 2 intermediates in the TCA cycle does FAD become FADH2?

Answer 3

Succinate and Fumerase

Question 4

What compound is joined with Acetyl-CoA to make citrate in the TCA cycle?

Answer 4

Oxaloacetate

Question 5

In the TCA cycle, what is fumerate hydrated to become?

Answer 5

Malate

Question 6

What 8 molecules are created during the citric acid cycle? In order

Answer 6

1. Citrate
2. Isocitrate
3. a-Ketoglutarate
4. Succinyl CoA
5. Succinate
6. Fumerate
7. Malate
8. Oxaloacetate

Question 7

How many key regulatory points are there in the Krebs cycle? Where are they?

Answer 7

2
Isocitrate dehydrogenase
a-Ketoglutarate dehydrogenase

Question 9

What is the standard reduction potential?

Answer 9

A measure of a compounds affinity for electrons

Question 10

What is the final electron acceptor in the ETC? What is the reduction potential compared to the other complexes?

Answer 10

Oxygen, has the highest potential

Question 11

What are the 2 methods of transporting NADH into the mitochondria?

Answer 11

Glycerol 3-phosphate shuttle
Malate-aspartate shuttle

Question 12

What is the name of complex I in the electron transport chain?

Answer 12

NADH coenzyme Q oxidoreductase

Question 13

What is the name of complex III in the ETC?

Answer 13

CoQ-Cytochrome c Oxidoreductase

Question 14

What is the name of complex IV in the ETC?

Answer 14

Cytochrome c oxidase