Metabolism Flashcards

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1
Q

Photosynthesis is…

A

The process by which plants use sunlight to convert carbon dioxide into glucose.

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2
Q

what does the thylakoid contain? How does it help photosynthesis?

A

Contains pigments that absorb light energy —> light energy is used to power photosynthesis

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3
Q

What does the stroma do? One sentence.

A

Uses the products of the thylakoid’s reactions to FINISH photosynthesis and create glucose.

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4
Q

Light energy ______ water.

A

Oxidizes

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5
Q

What happens in the first stage of photosynthesis? Three steps.

A
  1. Light energy oxidizes water.
  2. Electrons are excited by light and make ATP.
  3. Electrons reduce NADP+ into NADPH.
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6
Q

What happens in the second stage of photosynthesis? One step.

A

The NADPH and ATP add CO2 to carbon molecules to create glucose.

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7
Q

What is a pigment?

A

A substance that absorbs light.

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8
Q

What are OTHER names for the first and second stages of photosynthesis?

A
  1. Light dependent reactions.
  2. Light independent reactions.
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9
Q

The _______ pigments will travel the farthest on chromatography paper, because ________.

A

Most polar, because polar molecules are attracted to the water.

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10
Q

A stack of thylakoids is called a…

A

Granum.

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11
Q

Where do stages 1 and 2 of photosynthesis occur?

A

Stage 1 - thylakoid. Stage 2 - stroma.

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12
Q

Photosystems II and I are light __________

A

Dependent reactions.

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13
Q

Summarize photosystem II (P680) in three points.

A

1 - Photolysis, light energy splits oxygen into H+ and O2, releasing electrons.
2 - light energy excites electrons held in the reaction centre
3 - electrons move down electron transport chain, producing ATP

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14
Q

Summarize photosystem I (P700) in three steps.

A

1 - electrons from pII end up in reaction centre of pi. Electrons are excited by light energy once again.
2 - excited electrons are captured by another ETC. reduce NADP+ to NADPH
3- products (ATP and NADPH) are transferred to stroma for Calvin Cycle.

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15
Q

Describe the three stages of the Calvin Cycle.

A

1 - RuBP + 3 CO2. Ends up as 6 PGA. Catalyzed by RuBisCo.
2 - 6 PGA + ATP + NADPH, ends up as 6 PGAL/GA3P). Building block for glucose.
3 - 1 PGAL leaves to make 1/2 glucose. Rest regenerates RuBP.

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16
Q

What happens during photorespiration?

A

RuBisCo incorporates O2 into RuBP instead of CO2. Wasteful, no useful product.

17
Q

What two factors determine whether RuBisCo will use O2 or CO2?

A

Low temp, high CO2 concentration = Calvin Cycle
High temp, low CO2 concentration = photorespiration

18
Q

Describe how each C3, C4, and CAM plants process O2/CO2.

A

C3: use RuBisCo to convert CO2 into PGA. Sensitive to photorespiration.
C4: CO2 physically separated from oxygen to improve CO2 bonding (leaf tissue layers).
CAM: carbon dioxide reserves creates to improve CO2 consisting to RuBisco.

19
Q

How many ATP are created in Aerobic Respiration vs Anaerobic?

A

38 ATP in aerobic respiration. 2 ATP in anaerobic, plus a toxic byproduct (lactic acid in animals, alcohol in yeast)

20
Q

Now the four stage of aerobic respiration.

A

Glycolysis, pyruvate oxidation, citric acid/Krebs cycle, electron transport chain

21
Q

Where does glycolysis occur? Is it aerobic or anaerobic?

A

Cytosol, anaerobic.

22
Q

Where does pyruvate oxidation occur? Is it aerobic or anaerobic?

A

Mitochondrial matrix, aerobic.

23
Q

Where does the Krebs cycle occur? Is it aerobic or anaerobic?

A

Mitochondrial matrix, aerobic.

24
Q

Where does the ETC occur? Is it aerobic or anaerobic?

A

Inner membrane of mitochondria, aerobic.

25
Q

What are the inputs/outputs of glycolysis?

A

Inputs: 1 glucose, 2 ATP
Outputs: 2 pyruvate, 4 ATP, 2 NADH

26
Q

What else can be used instead of glucose during glycolysis? How does it work?

A

Fats - remove glycerol from fatty acids (using lipases), glycerol becomes PGAL
Proteins - proteases break protein into amino acids. deaminases remove amino group, rearranged into pyruvate/acetyl CoA

27
Q

What are the inputs/outputs of pyruvate oxidation?

A

Inputs: 2 pyruvate
Outputs: 2 acetyl CoA (byproduct, 2 CO2, 2 NADH)

28
Q

What are the inputs/outputs of the citric acid/Krebs cycle?

A

Inputs: 1 acetyl CoA
Outputs: 2 CO2, 3 NADH, 1 FADH2, 1 ATP

29
Q

Describe how the electron transport chain functions.

A

NADH & FADH2 deliver electrons and drive ATP synthesis. Energy stored from electron transfer through the chain forms a proton gradient, energy stored in gradient is used to make ATP.

30
Q

What is the role of oxygen in the ETC?

A

Oxygen “mops up” excess electrons and protons to make water. If oxygen is not present, ETC will stop and ATP production will also cease.