Metabolimic And Epigenetics Flashcards
What is epigenetics?
Study of phenotypic trait variations that are caused by gene expression
What is an epigentic trait?
Stable trait that is inherentable and does not alter DNA sequence
What is DNA methylation
A methly group attaches to a C followed by a G
Can be inherited that doesn’t change DNA
This create cell/tissue specific changes
Can be influence by environment
What does methyl groups do?
Too many methyl groups you block the expression of the gene
Which region has a lot of methylation and why?
Promoter bc it has a lot of Cg repeats 1/10 instead of 1/100
How does methylation affect histones?
Pack them tighter
What does acetlyation do to histones
Makes them loss
What does royal jelly do (analogy)
Makes queens or workers
How do methylation assay work
- Start with DNA and need to be really good dna bc it this process cuts up a lot
- Add bisulfite that will change all unmethyled CG to U
- Look at dna see which sites are changed and which stayed at CG
What is the issue with methylation assay
Not all the cells are same and there are variation
We measure proportion not the just one cell
what is DNA methylation with Microarrays
allows us to check the proportion of methyl at each site at a proportion
Spits it out in beta values 0-1 to show percentage
What is a beta Value?
Use in MicroAssays
0 = non methyl
1 = is methyl
Data is giving 0-1 to show percentage at a given site
Microarray pros and cons
Pros cheaper and high output
Cons limited to pre selected targets
Next generation pros and cons
Pros - captures larger genome
Cons - expensive and need to know what cell you are pulling from
Where can you pull from and what test can you do for inherited DNA
Any tissue (non germ)
All tests
