Metabolic Testing Of Bacteria- Lab14-19 Flashcards
What does amylase test test for
Amylase
What is the medium for the amylase test
Starch agar
What is the reagent for the amylase test
Iodine ; it turns the agar brown
Describe the amylase test
Two plates with straight streaks on them
Describe A positive result of the amylase test
Clearing around the growth line because the exoenzyme digested the starch
What does the lipase test test for
Lipase
What medium does a lipase test use
Tributyrin agar (cloudy vs. clear agar)
Describe a positive result for the lipase test
Clearing around the grounds line; obvious contrast because it ate up the fat
What does a gelatinase test test for
Gelatinase
What is the medium used for gelatinase test
Gelatin agar at 22°C (incubated at cooler temperature than the others)
Describe a positive result for a gelatinase test
Clearing around the growth line
Name the tests for enzymes of respiration
Catalase test , oxidase test , and nitrate reduction test
What does a catalase test test for
Catalase
What medium does a catalase test use
No medium- hydrogen peroxide(substrate)
Describe A positive result for the catalase test
Bubbles because of the oxygen gas
What does the oxidase test test for
Cytochrome C oxidase
What medium does the oxidase test use
BBL dry slide oxidase slide with reagent
What reagent does the oxidase test use
Reagent built into card
Describe a positive result for the oxidase test
Purple spot on slide in 20 seconds
What does the nitrate reduction test test for
Nitrate reductase
What medium was use in day one for the nitrate reduction test
Nitrate broth
What medium was used in day two of the nitrate reduction test
Nitrate test - hydrogen sulfide and TDR
nitrate test - zinc
Describe a positive test result for the nitrate reduction test
Bubble-nitrogen gas
Purple-Brown color
Name the catabolism of carbohydrates tests
Study the charts from lab 17 and 18
Name the tests for exoenzymes
Amylase test , lipase test , gelatinase test
