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Lab Practical #2 > Metabolic Testing Of Bacteria- Lab14-19 > Flashcards

Metabolic Testing Of Bacteria- Lab14-19 Flashcards

1
Q

What does amylase test test for

A

Amylase

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2
Q

What is the medium for the amylase test

A

Starch agar

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3
Q

What is the reagent for the amylase test

A

Iodine ; it turns the agar brown

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4
Q

Describe the amylase test

A

Two plates with straight streaks on them

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5
Q

Describe A positive result of the amylase test

A

Clearing around the growth line because the exoenzyme digested the starch

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6
Q

What does the lipase test test for

A

Lipase

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7
Q

What medium does a lipase test use

A

Tributyrin agar (cloudy vs. clear agar)

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8
Q

Describe a positive result for the lipase test

A

Clearing around the grounds line; obvious contrast because it ate up the fat

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9
Q

What does a gelatinase test test for

A

Gelatinase

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10
Q

What is the medium used for gelatinase test

A

Gelatin agar at 22°C (incubated at cooler temperature than the others)

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11
Q

Describe a positive result for a gelatinase test

A

Clearing around the growth line

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12
Q

Name the tests for enzymes of respiration

A

Catalase test , oxidase test , and nitrate reduction test

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13
Q

What does a catalase test test for

A

Catalase

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14
Q

What medium does a catalase test use

A

No medium- hydrogen peroxide(substrate)

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15
Q

Describe A positive result for the catalase test

A

Bubbles because of the oxygen gas

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16
Q

What does the oxidase test test for

A

Cytochrome C oxidase

17
Q

What medium does the oxidase test use

A

BBL dry slide oxidase slide with reagent

18
Q

What reagent does the oxidase test use

A

Reagent built into card

19
Q

Describe a positive result for the oxidase test

A

Purple spot on slide in 20 seconds

20
Q

What does the nitrate reduction test test for

A

Nitrate reductase

21
Q

What medium was use in day one for the nitrate reduction test

A

Nitrate broth

22
Q

What medium was used in day two of the nitrate reduction test

A

Nitrate test - hydrogen sulfide and TDR

nitrate test - zinc

23
Q

Describe a positive test result for the nitrate reduction test

A

Bubble-nitrogen gas

Purple-Brown color

24
Q

Name the catabolism of carbohydrates tests

A

Study the charts from lab 17 and 18

25
Q

Name the tests for exoenzymes

A

Amylase test , lipase test , gelatinase test

Lab Practical #2 (8 decks)

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