Metabolic Processes Flashcards

1
Q

• Endoplasmic Reticulum

A

Carries materials through cell & aids in making proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

• Cell Membrane

A

Controls movement in/out of cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

• Golgi Body

A

Processes, sorts, delivers proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

• Mitochondria

A

converts organic material into energy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

• Mitochondrial DNA

A

descendants of old prokaryotes in cell= have own DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

• Endosymbiotic Theory

A

Bacteria turned into chloroplast

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

• Leber’s Syndrome

A

Genetic (mitochondria DNA) disorder that can cause blindness in some, but only minor vision loss in others

-ammino acid in protein in ETC changed-> less effective= affects cells with high ATP demand (i.e. eyes)-> weaken/die

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

• Cytoplasm

A

Supports and protects organelles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

• Nucleus-

A

controls chem. Rx, stores info for mitosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

• Nucleolus

A

Produces ribosome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

• Chromosome

A

contains genetic information

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

• Ribosome

A

builds protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

• Cytoskeleton & Microtules

A

maintains cell structure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

• Lysosome-

A
  • Digest macromolecules
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Peroxisome-

A

rids cell of toxins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

• Chloroplast

A

conducts photosynthesis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

• Vacuole-

A

Secretory, excretory, storage functions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

• Cell wall-

A

protects cell interior

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

• Prokaryotic Cell

A

Simple cell with circular DNA, small and simple ribosomes, and no organelles or nucleus.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

• Eukaryotic Cell-

A

Complex cell with nucleus, linear DNA, large and complex ribosomes, and other organelles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

o Glycoprotein-

A

Oligosaccharide (few sugars)

  • attatched to membrane protein
  • Functions- Cell recognition of immune cells, hormone receptors
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

o Phospholipid Bilayer

A

Polar heads (Phosphate) face out, non polar tails (Fatty Acids) face in

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

o Integral Protein

A
  • Usually spans width of bilayer, slides laterally
    • Moves things in/out of cell (channels,pumps)
  • Acts as enzyme (i.e. ATP Synthase)
  • Receptors (Hormone receptors)
  • Anchors (attaches to cytoskeleton)
  • Cell recognition (ID)
  • Intercellular joinings (join cells together)
24
Q

o Cholesterol

A
  • anchors phospholipids in place, reduces fluidity (OH group anchors phosphohead on one, NP chain anchors FA on another)
    • prevents crystallization of FA at low temp.
25
o Concentration Gradient
difference in concentration b/w 2 areas
26
o Passive Transport
- no energy required (Diffusion) • Greater [] gradient, shorter distance, larger SA, higher temp= greater diffusion rate • Simple Diffusion • Facilitated Diffusion- carrier protein carries solute across membrane • Osmosis- diffusion of water across semi-permeable membrane
27
• Isotonic solution
- [water] outside cell= [water] inside cell | - equal water moves in/out of cell
28
• Hypotonic solution
- more water outside cell | - Water moves into cell, cell swells
29
• Hypertonic solution
- more water inside cell | - water moves out of cell, cell shrivels
30
o Active Transport
energy required (low [] to high [])
31
o Bulk Transport
- movement of large quantities in/out of cell (i.e proteins) - requires energy - Exocytosis- Exit cell - Endocytosis- enter cell
32
Phagocytosis-
“cell eating”, membrane extends/surrounds molecule/cell & forms vesicle
33
• Pinocytosis
“cell drinking”, cell pinches in and forms vesicle
34
• Receptor Mediated Endocytosis
- molecule binds to receptor site on “pit”, pit deepens and forms vesicle - Inside of pit coated in protein clathrin
35
o Cystic Fibrosis
- genetic disease - Normally CFTR protein lets chloride ions leave mucus producing cells, taking water with it, resulting in thinner mucus - CFTR protein doesn’t allow chloride out of mucus producing cells. Therefore thick mucus blocks lungs and digestive system= hard to breathe, can’t digest. - Mucus rich source of nutrients for bacteria= frequent infections
36
Cellular Respiration
o C6H12O6 +6O2 -> 6CO2 + 6H2O +ATP | o Produces ATP for chem, mechanical, transport work
37
o Subtrate-level phosphorylation-
ATP formed directly
38
o Oxidative phosphoylation-
- ATP formed indirectly from high energy molecules
39
• Aerobic
Oxygen present. Most efficient (36 ATP)
40
• Glycolysis
- 2 ATP used, 4 ATP, 2 NADH made - Forms 2x 3C pyruvate - In cytoplasm
41
• Trans Rx
- Pyruvate joins with CoA-> Acetyl-CoA (2 x 2C) - 2 CO2, 2 NADH made - In matrix
42
• Kreb’s Cycle (Citric Acid Cycle)
- CoA comes off - join with oxaloacetate (4 C) to form citrate (6C) (cycle) - 5 C at Ketoglutarate, 4 C at Succinyl-CoA - Makes 2 ATP (GDP->GTP->ATP), 6 NADH, 2 FADH2, 4 CO2 - All C from glucose gone
43
• ETC & Chemiosmosis
- 1 NADH-> 6 H+-> 3 ATP - 1 FADH2-> 4 H+ -> 2 ATP - NADH Reductase-> Coenzyme Q-> Cytochrome B/C1 ->Cytochrome C -> Cytochrome C oxidase -> ATP Synthase - Energy to pump 2 H+ across inner membrane into inter membrane space as e- pass. Builds electrochem gradient - e- leaves cytochrome C oxidase with oxygen and H+, forming water. MUST HAVE OXYGEN TO LEAVE OTHERWISE ETC IS BLOCKED - 2 H+ re-enters matrxi via ATP synthase-> 1 ATP formed - NADH from glycolysis-> FADH2 b/c NADH can’t cross mitochondria membrane - 8 NADH, 4 FADH2 used, 32 ATP made
44
• Anaerobic
- No oxygen present | - inefficient, only done when necessary (2 ATP made) or by prokaryotes/ simple eukaryotes with no mitochondria
45
• Lactic Acid Fementation
- Glucose- Pyruvate (Glycolysis)-> Lactate - Uses 2 NADH - Done by eukaryotes (humans)
46
• Ethanol Fermentation
- Glucose- Pyruvate (Glycolysis)-> Ethanol - Uses 2 NADH, releases 2 CO2 - Done by prokaryotes (bacteria)
47
• Other Metabolic Pathways
• Proteins- become amino acids - a.a. a. go through deamination (removes NH3 group). - Alanine-> pyruvic acid-> pyruvate - Aspartic acid-> oxaloacetate - Glutamate-> ketoglutamate • Fats- Undergo beta-oxidation (breaks down FA into acetate & combine with CoA). Becomes Acetyl-CoA - Glycerol becomes G3P * Hormones- become succinate * Cholesterol- becomes Acetyl-CoA * Nucleic Acids- Glutamate-> Ketoglutamate
48
o Chlorophyll
- green pigment that absorbs light and begins photosynthesis o Porphyrin Ring- Part of chloryphyll -Mg atom surrounded by hydrocarbon ring -Contains electrons that absorb light energy o Phytol Tail- Hydrophoic tail anchors chloryphyll to a membrane
49
o Absorption Spectrum
graph illustrating wavelengths of light absorbed by pigment
50
o Accessory pigments
pigments that work with chloryphyll a to absorb photons that chloryphyll a can’t i.e chloryphyll b, carotenoids
51
o Chromatography
a way of separating a mixture of pigments
52
• Non-Cyclic Light Rx
- Light hits PSII (680), excites electrons from chlorophyll a, electrons passed on to primary electron acceptor (PQ-piastoquinone) -Z protein splits water into oxyegn, H+, electrons. H+ builds electrochem gradient, electrons replace ones lost in PSII - PQ transfers 2 H+ across thylakoid membrane. Electrochem gradient builds - At the same time, light strikes PSI. Electrons release. Replaced by electrons from PC (plastocyanin) - Electrons move to Fd (ferrodoxin) and FNR (NADP reductase). NADPH produced - ATP Synthase makes ATP (4 H+ = 1 ATP - PSII->PQ->Cytochrom B6f->Pc->PSI->FD->FNR-> ATP synthase - Done by plants and algae - Uses pigments a,d,phycobilins
53
o Cyclic Light Rx
- Light strikes PSI (700), electrons travel from rx center to FD, Cytochrome b6f (pumps hydrogen across membrane) - electrons passed to Pc, returns to PSI, cycle repeats - Proton electochem gradient used to make ATP via ATP Synthase - PSI->FD->Cytochrom B6f->Pc->Repeats - Used by bacteria and plants - Uses pigments a,b,carotenoid,xanthophylls,anthocyanins
54
o Light Independent Reactions (Calvin Cycle)-
- Occurs in stroma o Phase 1 (Carbon Fixation)- RuBP and CO2 join to form 2 PGA (catalyzed by enzyme Rubisco o Phase II (Reduction)- PGA-> BPG via ATP breakdown BPG-> G3P via NADPH breakdown - 6 ATP used - 6 G3P forms, 1 leaves o Phase III (Regeneration of RuBP)- 5 G3P-> 3 RuBP -Uses 3 ATP
55
o Photorespiration
- Rubisco binds both oxygen and CO2. Increased temp= more O2 - turns oxygen into Co2 (like cell resp) - C4 Cycle- CO2 enters stomato, diffuses into mesophyll cell (No Rubisco). PEP Carboxolase (only binds CO2) fixed carbon dioxide to oxaloacete->malate/aspartate (transported to bundle sheath cell)-> CO2-> G3P - Bundle sheath cells are deep in leaf (hard for oxygen to reach), have thylakoids with reduced PSII (keeps O2 low). - CAM Plants- Intake CO2 at night (prevents water loss)->Oxaloacetic acid->malice acid->CO2->G3P