Metabolic Bone Disease - Histopathology Flashcards
The functions of bone
STRUCTURE – give structure and shape to the body • MECHANICAL – sites for muscle attachment • PROTECTIVE – vital organs and bone marrow • METABOLIC – reserve of calcium and other minerals
What are the five types of anatomical bones
Flat,long,short/cuboid - stabilisation ,irregular - protection of organs ,sesamoid- protective function
What are the two types of macroscopic bones
trabecular/cancellous/spongy
– cortical/compact
Cortical bone microanatomy
{add pic here}
What is the immature woven bone?
Disorganised, do not have structure, in developing skeleton, in situations with rapid bone and high bone turnover, very weak
What are the key bone cells?
Osteoclasts
Osteoblasts
Osteocytes
What are osteocytes?
Mechanosensory network embedded in mature bone
What are osteoclasts?q
Multi nuclear cells that resorb/remove bone
What are osteoblasts
Produce osteoid to form new bones
What is the bone remodelling cycle
[enter pic here] + add explanation
When does bone disease occur?
When there is excesss removal of bone by osteoclasts or excess increase in bone by osteoblasts.
Why perform a bone biopsy?
Confirm the diagnosis of a bone disorder
• Find the cause of or evaluate ongoing bone pain or
tenderness
• Investigate an abnormality seen on X-ray
• For bone tumour diagnosis (benign vs malignant)
• To determine the cause of an unexplained infection
• To evaluate therapy performance
What are the two types of bone biopsy?
Closed - needle - core biopsy (jamshidi needle)
Open - for sclerotic or inaccessible lesions
Transilliac bone biopsy
Typical location for bone biopsy bcs you can see all the different types of bone
What are some histological stains
- H&E
- Masson - Goldner Trichrome
- Tetracycline/Calcein labelling
