Mendelian Genetics Flashcards
What are the 5 reasons why the garden pea plant was an ideal organism for Mendels experiments?
- Easy to grow
- Short life cycle
- Large # of progeny
- Self-fertilizers
- Easy to cross-fertilize
How many different traits were individually analyzed during the pea plant experiments?
7
What are the 5 reasons mendels experiments were so successful?
- The garden pea is an ideal organism
- Traits were analyzed individually
- Pure-breeding lines were etablished (always same trait made)
- Reciprocal crosses performed (male purple x female white) and (male white x female purple)
- Performed statistical analyses to provide accurate conclusions
What is the law of segregation?
During gamete formation, each each pair of genes separate randomly so each gamete receives either with equal likelihood.
What is a test cross?
crossing unknown genotype w/ homozygous recessive to reveal genotype.
What is the definition of a dihybrid cross?
A cross between 2 hybrids differing in 2 traits.
What is the law of independent assortment?
Genes on different, nonhomologous chromosomes separate independently of one another during meiosis.
what is the binomial expansion equation and what are each of the variables related to?
P= n! / x! (n - x)! * p^x * q^n-x
n - total # of events ! - factorial x - # of events one category q - probability one category p - probability other category
Describe the process for performing the Chi-Square (X^2) Test. what is a null hypothesis? What does the p value indicate?
- Null hypothesis - No significant difference between expected and observed results.
- List each phenotype and # of observed
- calculate number of expected for each phenotype
- Perform X^2 analysis: [(observed - expected)^2 / expected
- determine degrees of freedom (df): N - 1 (N= # of classes)
- Find the p-value using df and X^2 value.
The P value indicates how accurate the null hypothesis is. if its greater than 0.05, there is greater than 5% chance that the null is accepted and the deviation is likely due to chance.
If the p-value is less than 0.05, it is less than 5% likely that the deviation is due to random chance. Therefore the null hypothesis can be objected.