Memory Flashcards
What did Hebb mean by a cell assembly?
If the inputs to a system cause the same pattern of activity to occur repeatedly, the set of active elements constituting that pattern will become increasingly strongly interassociated. That is, each element will tend to turn on every other element [..]. To put it another way, the pattern as a whole will become ‘auto- associated’. We may call a learned (auto- associated) pattern an engram”
In simple terms he was stating that“Cells that fire together, wire together”
Define briefly the term “long term potentiation” or “LTP”
Long term potentiation is the activity dependent, sustained increase in synaptic strength between neurons. Long term potentiation (LTP) was first described in the hippocampus by Bliss and Lomo in 1973. Their seminal studies resulted in the now widely accepted belief that this increase in synaptic strength is the cellular correlate of memory formation.
LTP can otherwise be worded as an increase in the synaptic response to a baseline stimulus. This increase is usually seen following tetanic stimulation (a burst of high frequency stimulation, usually at 100 Hz). As AMPA receptors are the main drivers of excitatory transmission, this usually equates to increased AMPA receptor insertion and increased AMPA receptor phosphorylation, resulting in an increased AMPA receptor conductance.
Explain the “classic” mechanism behind LTP. How does this fulfil one of Hebb’s postulates?
“Classic” LTP requires the activation of the NMDA receptor (NMDAR) on the postsynaptic membrane, which opens a channel to allow Ca2+ ions to enter the cell. The NMDAR acts as a coincidence detector of presynaptic and postsynaptic activity, as the channel opening requires not only the binding of glutamate (released by the presynaptic cell” but also the removal of the Mg2+ block which only occurs when the postsynaptic cell depolarises. This property allows NMDAR-dependent LTP to fulfil Hebb’s postulate, which stipulated that the connection strength between two neurons should increase if the presynaptic neurons continually causes the postsynaptic neuron to fire. This is summarised by his maxim that “cells that fire together wire together”(Hebb, 1949).
Why is it thought that memory formation requires protein synthesis?
It was initially demonstrated that behavioural memories are abolished by protein synthesis blockers by Flexner et al., 1963. As a result it was thought that memory formation must require protein synthesis. Only recently however, it has been found how protein synthesis contributes to memory formation. Various studies have delineated temporal phases of LTP. It is thought that LTP has an early phase, which is independent of protein synthesis and a late phase which requires it. The late phase of LTP has been further subcategorised into two stages by Kelleher et al in 2004. Their experiments which compare the kinetic effects of transcriptional (actinomycin D) and translational blockade (anisomycin) on L-LTP confirmed this temporal dissociation, defining an early translation-dependent, transcription-independent phase of L-LTP during the initial 60–90 min following induction and a subsequent transcription- and translation-dependent phase of L-LTP.
What is thought to underlie the two stages of L-LTP?
LTP2 is transcription-independent and translation-dependent, whereas LTP3 is transcription-dependent and translation-dependent.
The explanation for this difference in the inhibition kinetics is that the early effect of translational inhibition reflects the rapid translation of preexisting mRNAs, while the delayed effect of transcriptional inhibition reflects the time needed for the synthesis, processing, transport, and translation of newly synthesized mRNA.
(Kelleher, et al, 2004)
Is protein synthesis required for memory consolidation and reconsolidation as well as formation of memory (L-LTP)?
Agnihotri et al in 2004 showed that blocking protein synthesis in the brain of mice does not affect short-term stability of newly formed hippocampal place fields but abolishes stability in the long term. As a result inhibiting protein synthesis prevents the consolidation of place cell maps. This shows that place fields parallel the late-phase of long-term potentiation in requiring the synthesis of new proteins for consolidation.
However their experiments showed that inhibitingprotein synthesis does not affect the recall of previously established fields in a familiar environment, indicating that recall and therefore reconsolidation is not protein synthesis-dependent.
What are the proteins synthesised during memory formation and consolidation used for?
This protein synthesis is for building dendritic spines! This was demonstrated by Engert and Bonhoeffer in 1999. They combined a local superfusion technique with two-photon imaging, and showed that after induction of long-lasting (but not short-lasting) functional enhancement of synapses in area CA1, new spines appear on the postsynaptic dendrite, whereas in control regions on the same dendrite or in slices where long-term potentiation was blocked, no significant spine growth occurred.
What is a criticism of the synaptic theory of memory? (That memory is formed from LTP)
A criticism of the synaptic theory of memory is that dendritic spines how constant turnover. Spine numbers are affected by overall sensory input levels, motor activity, even the estrous cycle in female rats. It is still not clear how, given these processes, the ‘engram’ can be located in dendritic spines. However it has been shown that neocortical dendritic spines have a heightened permanency relative to those in the hippocampus (Yang et al., 2009), which gives an explanation as to why spine turnover in the hippocampus does not result in memory loss.
Is there evidence linking dendritic spines and memory?
A strong relationship between dendritic spine density in the hippocampus and memory has been demonstrated using different behavioral assessments. The acquisition of new memories in a conditioning paradigm is associated with increased spine density in CA1 pyramidal cells in adult rats (Leuner et al., 2003). Performance on two different spatial memory tasks, the Morris water maze and object placement, is associated with a higher dendritic spine density on pyramidal cells in CA1 (Moser et al., 1994) suggesting that there is a morphological substrate for memory.In addition, existing spines in the hippocampus undergo structural alterations that result in LTP (Jedlicka et al., 2008, Leuner et al., 2003). However it should be noted that at present it is impossible to determine whether increased dendritic spine density is the cause or result of improved hippocampal dependent memory in the aforementioned animal studies.
There is variability in how long LTP lasts. What evidence is there that L-LTP may involve more than one set of mechanisms that determine its persistence?
As previously stated, the early phase of LTP- LTP1 is protein-synthesis independent, whereas the late phase of LTP is protein synthesis dependent. L-LTP can be further subcategorised into LTP2 and LTP3. LTP2 is the early translation-dependent, transcription-independent phase of L-LTP and LTP3 is the subsequent transcription- and translation-dependent phase of L-LTP. This is shown by LTP decay curves by Abraham and Otani in 1991 demonstrating that LTP may involve more than one set of mechnaims that detremine its perisistence. (LTP1 decays faster than LTP2 and LTP2 decays faster than LTP3).More recently these mechanisms have been identified and shown by (Kelleher, et al, 2004).
What can explain the variablity in the peristence of LTP apart from how the differences in protein synthesis?
Many things must affect it but lecturer is going to focus on the NT dopamine.
In 2003, Li et al showed that exposure to a novel spatial environment promoted long term potentiation (LTP) at CA1 synapses in the rat hippocampus. They found that the brief exposure lowered the threshold for the induction of LTP but this was dependent on the activation of D1/D5 receptors. These findings support an important role for dopamine-regulated synaptic plasticity in the storage of unpredicted information in the CA1 area.
Dopamine is also thought to modulate memory consolidation. McNamara et al., 2014 found using an optogenic technique to stimulate VTA DA neurons that project to the hippocmapus in mice exploring novel environments improved the later recall of neural representations of space and stabilized memory performance. One theory of how dopamine contributes to improving memory recall is the synaptic tagging and capture theory (Frey & Morris, 1997. It is thought that dopamine may “tag” the synapse so that when proteins are synthesized they can be captured by the tagged synapse and used to stabilize the early plasticity changes allowing consolidation to occur.
Explain the terms memory consolidation and systems consolidation?
Memory consolidation refers to the process by which a temporary, labile memory is transformed into a more stable, long lasting form. For example, the term is commonly used to describe events at the synaptic/cellular level (e.g., protein synthesis and LTP), which stabilize synaptic plasticity within hours after learning. In contrast, systems consolidation, refers to gradual reorganization of information in the hippocampus to the neocortex, and is a process that occurs within long-term memory itself (Squire and Alvarez, 1995). Early evidence for systems consolidation was provided by studies of retrograde amnesia, which found that damage to the hippocampus-impaired memories formed in the recent past, but typically spared memories formed in the more remote past. E.g. patient HM showed retrograde amnesia for events in his 20s, but not from his childhood.
Briefly describe the two models that predict the necessity of the hippocampus for retrieval of remote memories.
The Standard Model of Systems Consolidation (SMSC) and Multiple Trace Theory (MTT)
The first is the Standard Model of Systems Consolidation (SMSC: Squire and Alvarez, 1995), a widely influential view in the field. The SMSC holds that the initial memory trace is encoded both in the hippocampus and in the cortex, though the cortex is itself unable to initially support the memory. Rather, the hippocampus is critical in early encoding stages. As a function of time, replay, and retrieval, the hippocampus “teaches” the cortex the memory trace such that the associative connectivity between the individual elements of the cortical memory increase in strength over time. After the memory has been consolidated, the hippocampus is no longer required for retrieval. This is based on the large body of evidence that synapses change much more rapidly and dynamically in the hippocampus than they do in cortex (Yang et al 2009). Thus, the SMSC predicts that the hippocampus is not required for the retrieval of remote memories, only recent ones that have not yet been consolidated.
The competing theory, known as Multiple Trace Theory (MTT), was proposed by Nadel and Moscovitch (1997) as an alternative to the standard model. Unlike the SMSC, MTT proposed that the hippocampus has an important role in the retrieval of all episodic memories, including remote ones. Similar to the SMSC, MTT also proposed that memories are encoded in hippocampal-neocortical networks, but that each reactivation resulted in a different trace in the hippocampus. Hippocampal-bound traces are presumed to be contextual and rich in spatial and temporal details, while cortical-bound traces are presumed to be semantic and largely context-free. Thus, retrieval of remote semantic memories does not require the hippocampus, however, retrieval of remote episodic memories always does, irrespective of the age of the memory.
Evidence for and against Standard Model of Systems Consolidation (SMSC)
Aspects of hippocampal lesion-induced amnesia can be found in Pavlovian fear conditioning, in which a tone conditional stimulus (CS) is paired with a shock unconditional stimulus (US) several times in a novel context. Rats trained in this manner develop a fear of both the tone and training context, which we measure as freezing, an adaptive species-specific defense reaction.
Contextual fear acquisition is thought to involve spatial learning (Nadel and Willner, 1980), and therefore support hippocampal involvement in contextual fear conditioning.
Kim and Fanselow (1992) gave rats tone-shock pairings before the animals received a lesion of the hippocampu. In this study, hippocampal lesions made 1 day after training produced a near-complete deficit in contextual fear, while sparing tone freezing. In contrast, lesions made 28 days after training failed to produce a reliable deficit. Hippocampal lesions produced a selective and time-limited retrograde amnesia of contextual fear. This led us to suggest that the hippocampus plays a temporary role in the formation of some aspects of contextual fear memory, which must be- come independent of the hippocampus over some time period after training.
As lesioning is an extremely invasive technique, which may produce damage in non-lesioned areas, and is permanent (no possibility to run a within-subject, baseline-manipulation-baseline design). Can optogenetics give us a new perspective?
Interestingly, it is important to note that recent studies have raised the possibility that even remote memory (memories that have consolidated to other brain regions) remains hippocampus-dependent (Goshen et al. 2011). This study does not support the standard model of systems consolidation.
It is assumed that memory “stored” in the hippocmapus is vulnerable to disruption but is safe when “stored” in the neocortex. Give an example where this has been shown not to be the case.
It was shown ex vivo that engram cells from the dentate gyrus established preferential connections with engram cells in the downstream hippocampal CA3 region. Remarkably, this preferential connectivity was maintained in mice rendered amnesic by treatment with PSI within the consolidation window, suggesting that memory storage may survive retrograde amnesia in the form of a neural connectivity pattern. Ryan et al 2015