Mediums and Reactions

Question 1

Mannitol Salt Agar

Answer 1

(Differential- Phenol Red- yellow below 6.8, red 7.4, pink above 8.2)
(Differential- Mannitol- Staphylococci ferment mannitol make acid end product- medium turn yellow)
(Selective- Staphylococci halotolerant- NaCl prevents others from growing)

Complex

Question 2

Pseudomonas Isolation Agar

Answer 2

(Complex Medium- selective only)
(Glycerol, magnesium chloride, potassium sulfate, triclosan-disinfectant)
(Pseudomonas aeruginosa- produce blue-green dye pigment pyocyanin)
(Pseudomonas fluorescnes- prod. yellow-green pigment flourescent under ultra violet light)