Medical Applications of Tissue Processing Flashcards
What are the 7 steps used to prepare a slide?
1- Fixation - formalin glutaraldehyde 2- Dehydration - graded series of EtOH 3- Clear - XYLENE 4- Embedding - PARAFIN 5- Sectioning 6- Mounting 7- Stain
Fix Da Clear Bed South of the Mountain Side (picture snow white in a clear bed and the dwarves fixing her glass bed)
Eosin/Hematoxylin - color and acid/base type
Eosin: Pink - acid dye - mitochondria, secretory vessicles, lysosomes, type I collagen
Hematoxylin: blue/purple - base dye - DNA, RNA, ribosomes, rER, sER,
HeManOx - DNA pulling oxcart instead of an ox. Men are blue
*****Feulgen Reaction
That video game guy - robot because he has no
- **DNA
- **Red-violet color
Mallory Triple
Trichrome - one dye does three different colors
Red - nuclei
Orange - muscles (red to orange)
Blue - collagen and hyaline
PAS Reaction
Polysaccharides - magenta!
Osmic Acid
Lipids - Black
Verhoeff
Backoff - pulling cartoon person off stage with hook, elastic body comes later; stages are black
Specific to ELASTIC fibers - Black
Trypan Blue
Blue
Ingested by macrophages, which are usually hard to see
Nissl
Nissan - Ride-a-some
Ribosomes
Blue
Iron Hematoxylin
Iron
Dark blue to black
Prussian Blue
Used to visualize iron in tissues
Blue
Congo Red
Identifies increased amyloid deposition in wall of blood vessel
Goes from red to red-green under polarized light
Very specific for amyloid protein
Marfan Syndrome
Thinning of elastic layer
Elastin - Verhoeff - black
Direct vs Indirect Immunochemistry
Direct: antibody given to see if adheres to sought-after protein; let it fluoresce
Less costly, less sensitive
Indirect: antigen binds to antibody binding to sought-after protein
Amplifies signal, more costly, more sensitive
Anticytokeratin Immunoperoxidase Technique
ID’s type of epothelial cancer
Cytokeratins - brown
Generally - assures epithelial origin
Specifically - aids in differentiation of cancer
