Media/reagents

Question 1

AIA medium

Answer 1

Actinomycete isolation agar, for isolating actinomycetes from soil due to starch, casein that others cannot hydrolyze as easily, has sodium propionate to discourage mold growth

Question 2

APT

Answer 2

All-purpose with tween media, for optimal enrichment and isolation and growth of lactic acid bacterial has oleic acid and other growth factors needed by fastidious lactic acid bacteria

Question 3

MacConkey agar

Answer 3

to detect and isolate gram-negative organisms, especially enterics and Pseudomonas, use bile salts and crystal violet to inhibit gram-positive bacteria growth

Question 4

Nutrient broth/agar

Answer 4

all purpose medium for organisms who are not nutritionally fastidious, does not have fermentable carbohydrates

Question 5

Succinate medium

Answer 5

used to cultivate anoxygenic anaerobic phototrophs

Question 6

Mossel EE broth

Answer 6

used to enrich Enterobacteriaceae and has dextrose (supports the growth of all enterics), has brilliant green and oxgall to inhibit gram-positive organisms, must not be shaken