Media overview Flashcards
TSA
-complex media
-non-selective and non differential
-growth
MSA
-Gram positive high salt;mannitol fermentation
-Mannitol fermenter should have growth with yellow medium
-mannitol non fermenter should have grwoth with red medium
-salt intolerance shoul dhave no growth
Selective and differential
BAP
-Gram positive (blood hemolysis)
-beta hemolytic - clearing
-alpha hemolytic - greening
-gamma hemolytic - no clearing
EMB
-gram negative lactose fermentation
-rapid lactose fermenter: metallic green/black
-slow lactose fermenter - fish eye/pink/brown
-non fermenter - pale and colourless
MAC
-selective and differential
gram negative lactose fermentation, selects for enteric
-strong lactose fermenter - matte pink with halo
-weake lactose fermenter - gelatinous pink no halo
-non - ferementer - pale
MRS
-selective for lactobacillus
-lactobacillus growth - white to yellow
BSM
-differential
-growth can take up to three days
-selective for bifidobacterium
-bifidobacterium growth - light to dark pink/brown
starch agar
-gram positive (starch hydrolysis)
-growth - non selective,
-differential
PCA
-complex media
-growth
-non selective
-non differential
KF
-fecal streptococci
-e. faecalis growth
-fermentation (of sugars causes acids to change bromocresol purple to turn yellow
-selective
-pH indicator bromocresol purple
-contains azide to inhibit aerobic organisms
complex medium
-large amount of variety of nutrients to accomodate the growth of numerous typles of bacteria regardles of nutritional requirements
-not chemically defines - had unknown composition of some ingredients
-TSA and PCA
-non specific
defined medium
-is chemically defined with a known composition of all components required to allow bacteria to grow
-non specific
non selective
-do not select for certain organisms
non differential
-do not use biochemical properties for differentiating bacterial growth
minimal media
-only a few chemically defined ingredients and does not promote a wide attay of microbes
