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Clinical Microbiology > Media > Flashcards

Media Flashcards

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Biology 101 flashcards
0
Q

NA

A

Nutrient Agar

Enriched

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1
Q

SBA

A

Sheep Blood Agar

Enriched

Diff: hemolysis
Alpha - green zone
Beta - clear zone
Gamma - no zone

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2
Q

PEA

A

Phenyl Ethyl Alcohol Agar

Selective: G+

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3
Q

MSA

A

Mannitol Salt Agar

Selective: salt tolerant

Differential: mannitol fermentation
+ yellow
- pink

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4
Q

MAC

A

MaConkey Agar - mildly inhibitory (G+)

Bile salts, crystal violet

Selective: G-

Differential: lactose fermentation
+ red
- colorless

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5
Q

TM

A

Thayer Martin

Selective: Neisseria gonorrhoae, Neisseria meningiditis

CO2

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6
Q

CHOC

A

Chocolate Agar

Enriched - fastidious

CO2

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7
Q

EMB

A

Eosin Methylene Blue - mildly inhibitory (G+)

Eosin Y, methylene blue

Selective: G-

Differential: lactose fermentation
+ yellow
- red

E. coli
+ metallic
- non-metallic

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8
Q

BG

A

Bordet-Gengou

Selective: Bordetella spp.

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9
Q

SS

A

Salmonella-Shigella Agar - moderately inhibitory (G+, coliforms)

Bile salts, brilliant green

Selective: Salmonella and Shigella

Differential: H2S production
+ black center
- other

Lactose fermentation
+ yellow
- red

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10
Q

HE

A

Hektoen Enteric Agar - moderately inhibitory (G+, coliforms, Proteus)

Differential: lactose fermentation
+ orange to salmon
- other

Salmonella spp.
+ blue to blue-green
H2S +

Shigella spp.
+ green

P. mirabilis
+ greenish
H2S +

Proteus spp.
Small green colonies
H2S -

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11
Q

Desoxychocolate agar

A

Desoxychocate agar - mildly inhibitory (G+)

Sodium desoxichocolate

Selective: G-

Differential: lactose fermentation
+ yellow
- red

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12
Q

XLD

A

Xylose-Lysine-Desoxychocate Agar - moderately inhibitory (G+, some coliforms)

Sodium desoxychocolate

Differential: H2S
+ black center

Coliforms and some Proteus spp.
Yellow colonies

Proteus spp.
Colorless colonies, H2S+

Salmonella spp.
Red colonies, H2S+

Shigella spp.
Colorless coined

Citrobacter spp.
Yellow colonies, H2S+

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13
Q

DC

A

Desoxychocolate Citrate Agar - moderately inhibitory (G+, coliforms)

Sodium desoxychocolate

Differential: lactose fermentation
+ pink colonies
- translucent to opaque colonies

H2S
+ brown center

Salmonella spp.
H2S+

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14
Q

BGA

A

Brilliant Green Agar - strongly inhibitory (G+, most G-, Salmonella typhi)

Brilliant green

Selective: Salmonella spp.

Differential: Salmonella spp.
+ pink-white colonies with red halo
- yellow-green colonies with yellow-green halo

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15
Q

BS

A

Bismuth Sulfite Agar - strongly inhibitory (G+, most G-)

Selective: Salmonella spp.

Differential: S. typhi, S. paratyphi B, S. enteritidis
Back colonies with metallic sheen

Other Salmonella spp.
Dark green colonies, H2S+/-

16
Q

CIN

A

Cefsulodin-irgasan-novobicin Agar - strongly inhibitory (G+, most G-)

Cefsulodin, irgasan, novobicin

Selective: Yersinia spp.

Differential: Y. entericolitica, other Yersinia spp., and some Citrobacter
Colonies with deep red centers and translucent periphery

Others
Pink colonies without red centers

17
Q

Mildly inhibitory

A

EMB
MAC
Desoxychocolate Agar

18
Q

Moderately Inhibitory

A

XLD
DC
SS
HE

19
Q

Strongly Inhibitory

A

BGA
BS
CIN

Clinical Microbiology (15 decks)

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