Media Flashcards
NA
Nutrient Agar
Enriched
SBA
Sheep Blood Agar
Enriched
Diff: hemolysis
Alpha - green zone
Beta - clear zone
Gamma - no zone
PEA
Phenyl Ethyl Alcohol Agar
Selective: G+
MSA
Mannitol Salt Agar
Selective: salt tolerant
Differential: mannitol fermentation
+ yellow
- pink
MAC
MaConkey Agar - mildly inhibitory (G+)
Bile salts, crystal violet
Selective: G-
Differential: lactose fermentation
+ red
- colorless
TM
Thayer Martin
Selective: Neisseria gonorrhoae, Neisseria meningiditis
CO2
CHOC
Chocolate Agar
Enriched - fastidious
CO2
EMB
Eosin Methylene Blue - mildly inhibitory (G+)
Eosin Y, methylene blue
Selective: G-
Differential: lactose fermentation
+ yellow
- red
E. coli
+ metallic
- non-metallic
BG
Bordet-Gengou
Selective: Bordetella spp.
SS
Salmonella-Shigella Agar - moderately inhibitory (G+, coliforms)
Bile salts, brilliant green
Selective: Salmonella and Shigella
Differential: H2S production
+ black center
- other
Lactose fermentation
+ yellow
- red
HE
Hektoen Enteric Agar - moderately inhibitory (G+, coliforms, Proteus)
Differential: lactose fermentation
+ orange to salmon
- other
Salmonella spp.
+ blue to blue-green
H2S +
Shigella spp.
+ green
P. mirabilis
+ greenish
H2S +
Proteus spp.
Small green colonies
H2S -
Desoxychocolate agar
Desoxychocate agar - mildly inhibitory (G+)
Sodium desoxichocolate
Selective: G-
Differential: lactose fermentation
+ yellow
- red
XLD
Xylose-Lysine-Desoxychocate Agar - moderately inhibitory (G+, some coliforms)
Sodium desoxychocolate
Differential: H2S
+ black center
Coliforms and some Proteus spp.
Yellow colonies
Proteus spp.
Colorless colonies, H2S+
Salmonella spp.
Red colonies, H2S+
Shigella spp.
Colorless coined
Citrobacter spp.
Yellow colonies, H2S+
DC
Desoxychocolate Citrate Agar - moderately inhibitory (G+, coliforms)
Sodium desoxychocolate
Differential: lactose fermentation
+ pink colonies
- translucent to opaque colonies
H2S
+ brown center
Salmonella spp.
H2S+
BGA
Brilliant Green Agar - strongly inhibitory (G+, most G-, Salmonella typhi)
Brilliant green
Selective: Salmonella spp.
Differential: Salmonella spp.
+ pink-white colonies with red halo
- yellow-green colonies with yellow-green halo
BS
Bismuth Sulfite Agar - strongly inhibitory (G+, most G-)
Selective: Salmonella spp.
Differential: S. typhi, S. paratyphi B, S. enteritidis
Back colonies with metallic sheen
Other Salmonella spp.
Dark green colonies, H2S+/-
CIN
Cefsulodin-irgasan-novobicin Agar - strongly inhibitory (G+, most G-)
Cefsulodin, irgasan, novobicin
Selective: Yersinia spp.
Differential: Y. entericolitica, other Yersinia spp., and some Citrobacter
Colonies with deep red centers and translucent periphery
Others
Pink colonies without red centers
Mildly inhibitory
EMB
MAC
Desoxychocolate Agar
Moderately Inhibitory
XLD
DC
SS
HE
Strongly Inhibitory
BGA
BS
CIN
