media Flashcards
For antibiotic susceptibility test of most bacteria
Mueller’s broth
For the identification of Gram negative enteric bacilli based on the ability to produce LDC (lysine decarboxylase) / ODC (ornithine decarboxylase) / ADH (arginine dihydrolase)
Mueller’s broth
For the isolation of pathogenic spp of Neisseria like Neisseria gonorrhoeae and Mycoplasma hominis, and Ureaplasma urealyticum
New York City agar (NYC)
Used to determine whether an organism can reduce nitrate to nitrite or to glucose end products such as Nitrogen
Nitrate Reduction Broth
For the determination of a non fastidious organism from a fastidious organism
Nutrient Agar
For the differentiation of organisms based on their ability to oxidize or ferment carbohydrate
Oxidation-Fermentation medium (O-F)
Useful for culturing anaerobes
Peptone-Yeast Extract Glucose Broth (PYG)
For the isolation of Mycobacterium spp.
Dorset Egg medium
Petragnani medium American
Thoracic Society medium (ATS)
For the isolation of nontuberculous Mycobacteria like Mycobacterium avium
Wallenstein medium
For the selective isolation of Gram positive cocci and anaerobic Gram negative bacilli
Phenylethanol agar (PEA)
For isolation of Corynebacterium diphtheriae
Potassium Tellurite Blood Agar
For the isolation of Mycoplasma spp.
Pleuropneumonia-like Organism Agar (PPLO)
For the isolation of Bordetella pertussis
Regan Lowe agar
For the isolation and differentiation of
Salmonella and Shigella
Salmonella-Shigella agar (SSA)
For the recovery of aerobes and anaerobes
Schaedler agar
For enrichment and isolation of Salmonella spp.
Selenite broth
For differentiating nonfermentative bacilli
like Pseudomonas, Acinetobacter, Alkaligenes
Seller’s agar
For isolation of Mycoplasma spp.
Shepard’s A7-B agar
Useful in differentiating gram-negative enteric bacilli
Simmon Citrate Agar
For the isolation of Campylobacter spp.
Skirrow’s medium
It is useful in the differentiation of Streptococci.
It distinguishes Enterococcus spp. from group D streptococci
Sodium Chloride Broth, 6.5%
Primary isolation medium for Mycoplasma spp.
SP-4 Broth / Agar
For the isolation of Streptococcus pyogenes and Streptococcus agalactiae
Streptococcal Selective agar (SSA)
Transport
Stuart’s medium
Biphasic medium
Scott’s modified Castaneda and Thioglycolate broth
Enrichment
For isolation of Salmonella and Shigella spp. from stool samples
Tetrathionate broth
For isolation of pathogenic species of Neisseria
Thayer Martin agar
For the recovery of aerobes, anaerobes, microaerophilic, and fastidious microorganisms
Thioglycollate (Thioglycolate) broth
For isolation of Corynebacterium diphtheriae
Tinsdale agar
For isolation and differentiation of Vibrio spp.
Thiosulfate Citrate Bile Salts Sucrose
agar (TCBS)
For enrichment and isolation of Streptococcus
agalactiae
Todd-Hewitt broth supplemented with
antibiotics
Selective Transport For Neisseria gonorrhoeae
Transgrow
General Purpose Medium
Trypticase Soy broth / agar
For the identification of Gram negative enteric bacilli based on their ability to ferment glucose, lactose, and sucrose. Produce gas and hydrogen sulfide
Triple Sugar Iron agar (TSI)
For the identification of Gram negative enteric bacilli based on their ability to produce indole
Tryptone broth
Used for Indole Test in the identification of Enterobacteriaceae species and non glucose- fermenting, gram negative bacilli
Tryptone broth, 1%
For the identification of Gram negative enteric bacilli based on their ability to ferment glucose via mixed acid fermentation or butylene glycol pathway
Methyl Red Voges Proskauer broth (MRVP) or Peptone Glucose broth
For the identification of Gram negative enteric bacilli based on their ability to produce enzyme deaminase.
Phenylalanine Deaminase agar (PDA)
Useful in identifying species of Enterobacteriaceae
Urea Agar and Broth
Differentiates beta hemolytic Streptococci and Enterococci based on their ability to produce enzyme, pyrrolidonyl arylamidase or pyroglutamyl aminopeptidase
PYR agar
Isolates and identifies Pseudomonas aeruginosa
Cetrimide agar slant
McLeod’s agar
Pai’s agar
For the identification of Corynebacterium diphtheriae
For the isolation of Rickettsia
Hela Cell line
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