Media Flashcards
what is it necessary for bacteria to grow
water essential nutrient correct pH correct temperature correct gaseous environments
what temperature to avoid contamination
5
simple media/nutrient agar
basi nutrient for undemanding bacteria
enriched media
require additional ingredients
blood agar
5-10% blood , mammalian pathogens detect haemolysis (virulence)
type of haemolysis
b: complete destruction of RBC, clear area around the growth
a: partial destruction of RBC , greenish decolourisation in the agar
y: no destruction of RBC so no change
chocolate agar
blood agar heated at 80 degrees to rupture the RBC and release haemogoblin
enriched broths
liquid medium selective for different bacteria
selective media
nutritional requirments are only for a determied group of bacteria and colonies
MacConkey’s agar
crystal violet and bile salts
lactose-fermenting enteric bacteria (pink)
pH indicator
bile salt tolerant gram -ve bacteria (no +ve)
deoxycholate-citrate agar
inhibits no-enteric bacteria
growth of salmonella
EMB agar
isolation and differentiation of enteric bacteria
favours gram -ve bacteria
contains lactose
Brilliant green agar
inhibits gram +ve and some -ve bacilli
pH indicator
sabourands agar
ringworm
high glucose with low pH
fungal growth
