Mechanism of enzyme action Flashcards
what is an enzyme- product complex
enzyme molecule with products in active site, joined temporarily with non-covalent forces
what is an enzyme substrate complex
enzyme molecule with substrate molecules in its active site. Joined by temporary non covalent forces
what is the lock and key hypothesis
The hypothesis that the substrate fits into the enzyme active site precisely with temporary hydrogen bonds, to form an ES complex
who created the lock and key model
Emil Fischer
What proteins are enzymes
Globular, shape is determined by the tertiary structure
what is the induced fit model
Active site makes conformational changes to allow the substrate to bind to the enzyme and maximise enzyme catalysation
what comes first an EP Complex or an ES complex
ES
then EP
what happens to the amino acids in the active site needs to make conformational changes
Conformational changes cause the Active site’s R-groups change shape - allows a more precise shapefor the substrate
What bond is a non covalent force
Hydrogen bonds
What causes products to leave the active site
Products have a slightly different shape to the substrate so they can detach from the active site
What do enzymes do to the activation energy
They lower the activation energy
What is activation energy
the minimum amount of energy that must be available to reactants for a chemical reaction to occur
