mcq Flashcards
which of the following is the correct name for a widely used gene that metabolises X-gal and differentiates from non-recominant plasmids during bacterial cloning of recombinant DNA?
LacZ
which of the following bacterial species can be used to produce transgenic plants using recombinant Ti Plasmids?
Agrobacterium tumefaciens
What does the Ti stand for in Ti plasmid
Tumour-inducing
what percentage of transgenic offspring would be expected using transgenesis by transfer of transfected nuclei?
1-5%
Transgenic golden rice was developed to alleviate deficiency of which vitamin in certain human populations
Vitamin A
how many rounds of cell nuclear division take place in eukaryotic meiosis?
two
A coenocyte is…?
A multinucleated cell generated from multiple nuclear divisions without cytokinesis
the sheep haploid chromosome number is 27.ignoring recombination how many chromosomal combinations can result during meiotic cell division in horses?
134,217,728
which of the following is not a component of a typical PCR reaction?
DNA ligase
what is the theoretical number of polymerase chain reaction (PCR) amplicions expected after 35 cycles with a starting number of 20 target sequences?
20x2 to the power of 35= 687,194,767,360
which of the following is the correct name for a widely used gene that confers resistance to an antibiotic during bacterial cloning of recombinant DNA?
amp^R
what is the missing phrase. The chromatin in eukaryotic cell nuclei consists of___________
protein and deoxyribonucleic acid(DNA)
the dwarf phenotype in dexter cattle is associated with a lethal recessive allele. the leathal recessive condition is termed “bulldog” which gives rise to stillborn calves with severe facial deformities. Male dwarf dexter cattle are crossed with female dward dexter cattle. what would be the expected pheontypic proportions of the offspring produced?
50% dwarf cattles/ 25% normal-sixed calves/ 25% still born bulldog calves
what is the term used to describe the genotype of an X-linked gene in male mammals?
hemizygous
which of the following is the correct description of the ABO blood group system in humans?
The human ABO blood group system is controlled by a single gene with two codominant alleles and one recessive allele.
what is the correct definition of epistasis?
the action of one gene modifying the effects of other genes.
the restriction enzyme EcoRI cuts DNA molecules athe the following restriction site:
5’ GAATTC 3’
3’ CTTAAG 5’
how many double- stranded fragments will result if the following dna molecule is cut off at EcoRI?
two
which of the following terms relates to the structure of double-stranded DNA?
hydrogen bond
what is the function of the retroviral enzyme reverse transcriptase?
transcribes a DNA molecule from a single-stranded viral RNA template.
which of the following is correct in relation to DNA agarose gel electrophoresis?
DNA migrates from negative to the positive electrode.
what is the Sanger lab method used for?
Determining the sequence of bases in a DNA molecule.
the following diagram shows the temp profile for the start of a typical PCR. what are the correct temps of each stage of the pcr reaction?
temp A- 55.
temp B - 72
temp C - 94
what is the theoretical number of PCR amplicons expected after 40 cycles with a starting number of 10 target sequences?
10 x 2^40= 10,995,116,277,760
which of the following is not used to produce transgenic animals?
injection of dna into an animals blood circulatory system using a hydodermic needle.
what is missing.
the chromatin in eukaryotic cell nuclei consists of______?
DNA & protein
what is the correct definition of the term ‘cell cycle’
the series of events in a eukaryotic cell from one cell division to the next
what is the full acronym for mRNA
messenger ribonucleic acid
the biochemical process of translation is carried out by which molecule?
the ribosome
which of the following will most dramatically change the amino acid sequence of a polypeptide?
a single nucleotide deletion in the exon of a gene.
when snapdragon plants produce red flowers are crossed with snap dragon plants that produce white flowers the resulting offspring produce pink flowers. what is the genetic term used to describe this phenomenon?
incomplete dominance
who is considered to be the father of genetics?
Gregor Mendel
what is the correct description for a diploid organism with a pair of identical alleles for a character?
homozygous
the chromatin in eukaryotic cell nuclei consists of ________?
deoxyribonucleic acid and protein
?if mottled overo stallions mated with mottled overo mares what would the phenotypic proportions of foals born be
50% mottled overo foals. 25% solid colour foals. 25%white still born foals.
what does the r stand for in RNA?
ribosomal RNA
what correctly describes complimentarybase pairing in DNA?
a thymine base (T) is always base paired with adenine via 2 hydrogen bonds.
what term is used to describe the genotype of an x-linked gene in male mammals?
hemizygous
what is the correct term for the word pleiotrophy?
the genetic effect of a single gene on multiple phenotypic traits.
which of the following processes can enhance the evolutionary adaptation of a species to its environment?
natural or artificial selection
a colour blind woman has children with a man who has normal visions. what are the expected offspring?
50% carrier females 50% colour blind males
s wright, jbs haldane and ra fisher initiated which branch of genetics?
population
an evolutionary founder event will lead to?
increase genetic drift
