mcq

Question 1

which of the following is the correct name for a widely used gene that metabolises X-gal and differentiates from non-recominant plasmids during bacterial cloning of recombinant DNA?

Answer 1

LacZ

Question 2

which of the following bacterial species can be used to produce transgenic plants using recombinant Ti Plasmids?

Answer 2

Agrobacterium tumefaciens

Question 3

What does the Ti stand for in Ti plasmid

Answer 3

Tumour-inducing

Question 4

what percentage of transgenic offspring would be expected using transgenesis by transfer of transfected nuclei?

Answer 4

1-5%

Question 5

Transgenic golden rice was developed to alleviate deficiency of which vitamin in certain human populations

Answer 5

Vitamin A

Question 6

how many rounds of cell nuclear division take place in eukaryotic meiosis?

Answer 6

two

Question 7

A coenocyte is…?

Answer 7

A multinucleated cell generated from multiple nuclear divisions without cytokinesis

Question 8

the sheep haploid chromosome number is 27.ignoring recombination how many chromosomal combinations can result during meiotic cell division in horses?

Answer 8

134,217,728

Question 9

which of the following is not a component of a typical PCR reaction?

Answer 9

DNA ligase

Question 10

what is the theoretical number of polymerase chain reaction (PCR) amplicions expected after 35 cycles with a starting number of 20 target sequences?

Answer 10

20x2 to the power of 35= 687,194,767,360

Question 11

which of the following is the correct name for a widely used gene that confers resistance to an antibiotic during bacterial cloning of recombinant DNA?

Answer 11

amp^R

Question 12

what is the missing phrase. The chromatin in eukaryotic cell nuclei consists of___________

Answer 12

protein and deoxyribonucleic acid(DNA)

Question 13

the dwarf phenotype in dexter cattle is associated with a lethal recessive allele. the leathal recessive condition is termed “bulldog” which gives rise to stillborn calves with severe facial deformities. Male dwarf dexter cattle are crossed with female dward dexter cattle. what would be the expected pheontypic proportions of the offspring produced?

Answer 13

50% dwarf cattles/ 25% normal-sixed calves/ 25% still born bulldog calves

Question 14

what is the term used to describe the genotype of an X-linked gene in male mammals?

Answer 14

hemizygous

Question 15

which of the following is the correct description of the ABO blood group system in humans?

Answer 15

The human ABO blood group system is controlled by a single gene with two codominant alleles and one recessive allele.

Question 16

what is the correct definition of epistasis?

Answer 16

the action of one gene modifying the effects of other genes.

Question 17

the restriction enzyme EcoRI cuts DNA molecules athe the following restriction site:

5’ GAATTC 3’
3’ CTTAAG 5’

how many double- stranded fragments will result if the following dna molecule is cut off at EcoRI?

Answer 17

two

Question 18

which of the following terms relates to the structure of double-stranded DNA?

Answer 18

hydrogen bond

Question 19

what is the function of the retroviral enzyme reverse transcriptase?

Answer 19

transcribes a DNA molecule from a single-stranded viral RNA template.

Question 20

which of the following is correct in relation to DNA agarose gel electrophoresis?

Answer 20

DNA migrates from negative to the positive electrode.

Question 21

what is the Sanger lab method used for?

Answer 21

Determining the sequence of bases in a DNA molecule.

Question 22

the following diagram shows the temp profile for the start of a typical PCR. what are the correct temps of each stage of the pcr reaction?

Answer 22

temp A- 55.
temp B - 72
temp C - 94

Question 23

what is the theoretical number of PCR amplicons expected after 40 cycles with a starting number of 10 target sequences?

Answer 23

10 x 2^40= 10,995,116,277,760

Question 24

which of the following is not used to produce transgenic animals?

Answer 24

injection of dna into an animals blood circulatory system using a hydodermic needle.

Question 25

what is missing.

the chromatin in eukaryotic cell nuclei consists of______?

Answer 25

DNA & protein

Question 26

what is the correct definition of the term ‘cell cycle’

Answer 26

the series of events in a eukaryotic cell from one cell division to the next

Question 27

what is the full acronym for mRNA

Answer 27

messenger ribonucleic acid

Question 28

the biochemical process of translation is carried out by which molecule?

Answer 28

the ribosome

Question 29

which of the following will most dramatically change the amino acid sequence of a polypeptide?

Answer 29

a single nucleotide deletion in the exon of a gene.

Question 30

when snapdragon plants produce red flowers are crossed with snap dragon plants that produce white flowers the resulting offspring produce pink flowers. what is the genetic term used to describe this phenomenon?

Answer 30

incomplete dominance

Question 31

who is considered to be the father of genetics?

Answer 31

Gregor Mendel

Question 32

what is the correct description for a diploid organism with a pair of identical alleles for a character?

Answer 32

homozygous

Question 33

the chromatin in eukaryotic cell nuclei consists of ________?

Answer 33

deoxyribonucleic acid and protein

Question 34

?if mottled overo stallions mated with mottled overo mares what would the phenotypic proportions of foals born be

Answer 34

50% mottled overo foals. 25% solid colour foals. 25%white still born foals.

Question 35

what does the r stand for in RNA?

Answer 35

ribosomal RNA

Question 36

what correctly describes complimentarybase pairing in DNA?

Answer 36

a thymine base (T) is always base paired with adenine via 2 hydrogen bonds.

Question 37

what term is used to describe the genotype of an x-linked gene in male mammals?

Answer 37

hemizygous

Question 38

what is the correct term for the word pleiotrophy?

Answer 38

the genetic effect of a single gene on multiple phenotypic traits.

Question 39

which of the following processes can enhance the evolutionary adaptation of a species to its environment?

Answer 39

natural or artificial selection

Question 40

a colour blind woman has children with a man who has normal visions. what are the expected offspring?

Answer 40

50% carrier females 50% colour blind males

Question 41

s wright, jbs haldane and ra fisher initiated which branch of genetics?

Answer 41

population

Question 42

an evolutionary founder event will lead to?

Answer 42

increase genetic drift