MCQ Flashcards

1
Q

hazard

A
  • ability of a substance or biological agent to cause harm
  • out of control
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2
Q

risk

A
  • likelihood substance or biological agent might be harmful under specific circumstances
  • can be controlled
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3
Q

microbe hazard group one

A

unlikely to cause human disease

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4
Q

microbe hazard group two

A

may cause disease: possible hazard to lab workers, minimal hazard to community

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5
Q

microbe hazard group three

A

may cause severe disease: may be serious to lab workers and may spread to community

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6
Q

microbe hazard group four

A

causes severe disease: serious hazard to lab workers and high risk to community

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7
Q

tera (T)

A

10^12

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8
Q

Giga (G)

A

10^9

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9
Q

Mega (M)

A

10^6

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10
Q

Kilo (K)

A

10^3

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11
Q

hecto (h)

A

10^2

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12
Q

Deka (da)

A

10

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13
Q

deci (d)

A

10^-1

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14
Q

centi (c)

A

10^-2

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15
Q

milli (m)

A

10^-3

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16
Q

micro (u)

A

10^-6

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17
Q

nano (n)

A

10^-9

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18
Q

pico (p)

A

10^-12

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19
Q

femto (f)

A

10^-15

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20
Q

mole

A

6.02 * 10 ^23
number of atoms in 12g of carbon-12

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21
Q

intrinsic hazard

A

physical, chemical and biological

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22
Q

extrinsic hazard

A

how something used i.e amount used, procedures

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23
Q

colony forming units

A

(counts*dilution factor)/volume plated

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24
Q

dilution

A

moles= concvol
ca
va = cb*vb

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25
Q

accuracy

A

how close something is to the actual value

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26
Q

precision

A

how close something is to other values measured

27
Q

residuals

A

distance between fitted line and an observation

28
Q

least squares regression

A

move the line to minimise total of the squared residuals

29
Q

exponent >1 on graph

A

increasing returns

30
Q

exponent < 1 on graph

A

decreasing returns

31
Q

histogram

A

shows distribution of scores

32
Q

how minimise risk

A
  • clear instructions
  • safety info
  • risk assessments
  • PPE
  • fire alarm
  • well maintained lab equipment
  • first-aiders
  • trained demonstrators
  • emergency showers
33
Q

systematic errors

A
  • equipment
  • procedural
  • human
34
Q

random error

A
  • biological samples often variable
  • factors beyond control e.g. environment
  • use of repitition reduces
35
Q

R-squared

A

proportion of variation explained by the trendline
higher R- squared normally better

36
Q

occam’s razor/ The parsimony principle

A

when faced with competing hypotheses select one with fewest assumptions

37
Q

standard deviation

A

spread of values around their mean

38
Q

standard error

A

range of uncertainty for a value
normally shown by an error bar
standard deviation/ square root of sample size

39
Q

intensive properties

A

independent of amount

40
Q

extensive properties

A

depends on amount of substance

41
Q

Mann- Whitney U test

A

non-parametric test for difference between 2 samples
data in ranks

42
Q

non-parametric

A

data not normally distributed

43
Q

parametric

A

normally distributed

44
Q

correlation coefficient

A

measure strength of linear trend between two sets of independent data
r between -0.1 and 0.1 no trend
r about 0.3 (-0.3) call trend
r = +1 (-1) perfectly straight line

45
Q

p- value

A

likelihood of seeing a pattern at random

46
Q

critical value p<0.05

A

significant

47
Q

degrees of freedom

A

number of independent pieces of information used to calculate statistic
n-2

48
Q

stats test to identify trend or relation

A

regression
correlation

49
Q

regression stats test

A

trend with one variable manipulated and other dependent

50
Q

stats test to compare samples

A

Mann- Whitney U test
two sample t test
chi-sq test

51
Q

what label on petri dish

A

name
date
organism name
bench number

52
Q

chi-squared

A

frequencies of observations in different categories

53
Q

two sample t test

A

look for significant difference between distribution in 2 independent samples

54
Q

when counting CFUs how many should chose

A

30-300

55
Q

untis for CFU

A

ml^-1

56
Q

diluting conc acid should

A

get hot

57
Q

spill conc acid should

A

rinse thoroughly with water

58
Q

purpose of aseptic techniques

A

prevent contamination

59
Q

what allows DNA seen under UV light

A

ethidium bromide

60
Q

what colour flame used to sterilise equipment

A

blue

61
Q

what effects migration rate of DNA molecules during gel electrophoresis

A

length
shape
agrose concentration

62
Q

how to separate smaller DNA molecules from each other

A

increase agarose conc

63
Q

equality

A

ensuring that every individual has an equal opportunity to make the most of their lives and talents

64
Q

Mary Anning

A

exclusion due to gender class and religion