MCB10 - Sensing/Responding to Extracellular Matrix Flashcards
What shapes are cells that are in suspension.
Circle as they are not adhered to the surface.
What is required for cells to adhere to a surface.
Matrix molecules
Cell spreading is passive. True or false.
False. Cell spreading requires energy in order to adhere and detach. In addition, energy to required for polymerisation of cytoskeleton elements forming the lamellipods which aid movement.
What happens to cells that fail to attach to the matrix.
Cell death. Cannot receive appropriate signals and nutrients required for growth therefore will undergo apoptosis.
Define organoids.
Artificially grown masses of cells and tissue that resemble organs.
What conclusion can be made about cells surviving only when attached to matrix.
Cells must be able to sense their environment
What are the effects to cells given the following conditions:
- cell in suspension, no growth factor
- cell in suspension, yes growth factor
- cell attached to matrix, no growth factor
- cell attached to matrix, yes growth factor
1) no cell division, cell death
2) no cell division, cell death
3) no cell division, no cell death
4) cell division, no cell death
How do cells bind to ECM molecules.
Have receptors on their surface which are complementary to the ECM molecules e.g. cell matrix adhesion molecules
How do receptors involved with cell matrix adhesion attach to internal cytoskeletal elements.
Mechanism continuity is present through use of linker proteins
What are integrins and what is their role.
ECM receptors which link to the actin cytoskeleton via actin binding linker proteins. Cluster to form focal adhesions or hemidesmosomes and aid signal transduction.
