MC

Question 1

The final electron acceptor of the ETC that functions in oxidative phosphorylation is?

Answer 1

Oxygen

Question 2

Most of the ATP made during aerobic respiration is generated by?

Answer 2

Oxidative phosphorylation

Question 3

The direct energy source that drives ATP synthesis during oxidative phosphorylation is?

Answer 3

The difference in H+ concentration on opposite sides of the cytoplasmic membrane

Question 4

Which metabolic pathway is common to both fermentation and aerobic respiration

Answer 4

Glycolysis

Question 5

Which is not a common product of glycolysis, PPP, and Entner-Doudoroff pathway?

a. ATP
b. NADPH
c. pyruvate
d. G-3-P

Answer 5

b. NADPH

Question 6

The TCA cycle is important to bacteria because it can produce up to ___ ATP?

Answer 6

36

Question 7

For bacteria growing on acetate, which key pathway must they have in order to improve growth efficiency

Answer 7

Glyoxylate bypass

Question 8

The reason for cyanobacteria like anabaena to carry out nitrogen fixation in heterocysts is because it?

Answer 8

Protects nitrogenase from oxygen

Question 9

If ammonium sulphate was not provided in the growth medium, bacteria like E. coli would be most impeded in making which metabolite?

Answer 9

Amino acids

Question 10

in PEP + NAD+ –> pyruvate + NADH, which is the oxidizing agent?

Answer 10

NAD+

Question 11

Nitrogen fixation involves forming __ from ___

Answer 11

Formation of NH3 from N2

Question 12

When electrons flow along the electron transport chain in E. coli, which of the following changes occurs?

Answer 12

A PMF is generated

Question 13

Which of the following is a true distinction between respiration and fermentation with respect to NADH?

Answer 13

NADH is only oxidized by the ETC in respiration

Question 14

The process of CO2 fixation requires which compound(s)

Answer 14

ATP (9) and NADPH (6)

Question 15

In citrate fermentation, what is generated? By what mechanism?

Answer 15

NADH is generated by reverse electron flow

Question 16

True or false, the ATP from fermentation is derived from both substrate level phosphorylation and oxidative phosphorylation?

Answer 16

False

Question 17

What bacteria are most likely to have pyruvate ferredoxin oxireductase and not pyruvate dehydrogenase?

Answer 17

Anaerobic bacteria

Question 18

Which of the following is true about nitrification

a. nitrate is the terminal electron acceptor
b. ammonia is converted to nitrate in a 2 step process by 2 groups of bacteria
c. nitrite is produced as the final product
d. N2 is converted to NH3 by nitrogenase

Answer 18

b. ammonia is converted to nitrate in a 2 step process by 2 groups of bacteria

Question 19

What are the 3 forms of chemical energy

Answer 19

ATP, acetyl-CoA, acyl phosphates

Question 20

What is the structure of T3SS?

Answer 20

needle like structure that can cross 3 biological membranes

-injects effectors into host cells

Question 21

If glutamine synthase and glutamate dehydrogenase are inactive, what compound(s) can bacteria not make?

Answer 21

NH3 and NO3-

Question 22

P-type ATPases have the following characteristics except:

a. Ion uptake
b. heavy metal ion efflux
c. ATP hydrolysis
d. found only in gram-positive bacteria
e. integral membrane proteins

Answer 22

d. found only in gram-positive bacteria

Question 23

T/F ATP is generated by substrate level phosphorylation in photosynthesis?

Answer 23

False

Question 24

T/F ATP and NADPH can be generated by cyclic phosphorylation in photosynthesis?

Answer 24

False

Question 25

T/F the source of electrons for non-cyclic photophosphorylation is H20 in bacterial photosynthesis

Answer 25

True

Question 26

T/F all photsynthetic bacteria produce oxygen when carrying out photosynthesis?

Answer 26

False

Question 27

Which of the types of protein secretion system is not present in gram positive bacteria? a. Sec system b. TAT system c. cotranslational translocase d. Type -1 secretion

Answer 27

T1SS

Question 28

What do the proteins have that are secreted by the T2SS?

Answer 28

an N-terminal leader sequence

Question 29

Which of the following is not true of T4SS? a. translocate effector proteins into host cells b. export DNA to target cells c. secrete effector proteins into medium d. effector proteins pushed out by pseudopilus

Answer 29

d. effector proteins pushed out by pseudopilus

Question 30

T/F 6 protons are extruded across the membrane by the ETC if the electron donor is FADH2?

Answer 30

False, its 4

Question 31

T/F T4SS secreted effectors into host cells and results in actin rearrangement ?

Answer 31

False, T3SS does this

Question 32

T/F Ionophores can be used to measure ∆Psy because they form lipid soluble complexes with cations

Answer 32

True

Question 33

T/F Bacteria that produce bacteriorhodopsin can use it to generate a proton gradient?

Answer 33

true

Question 34

T/F Kreb cycle is important to bacteria because it provides the source for synthesis of 10 amino acids

Answer 34

True

Question 35

T/F Siderophores are small molecules that take in all metal cations

Answer 35

False

Question 36

T/F after an osmotic shock treatment, E. coli is no longer able to transport nutrients because it has lost the porin proteins?

Answer 36

False

Question 37

T/F proteins translocated by the TAT system are prefolded?

Answer 37

true

Question 38

T/F aerobic bacteria use organic intermediates from the metabolic pathway to re-oxidize NADH?

Answer 38

False (use ETC)

Question 39

T/F in non cyclic photophosphorylation, the initial electron donor is NADPH?

Answer 39

False

Question 40

T/F intramolecular disulphide bond formation is catalyzed by DbsA occurs in the cytoplasm

Answer 40

False, occurs in the periplasm

Question 41

T/F ABC transporters are involved in nutrient uptake and toxin export ?

Answer 41

true