Mbio Lab Test 2

Question 0

Reagents for VP test

Answer 0

Alpha napthol and K hydroxide

Question 1

Reagents for nitrate reduction test

Answer 1

Sulfanic acid and napthylamine

Question 2

Reagents for indole

Answer 2

Kovacs

pDMAB and isoamyl alcohol

Question 3

Ph indicator for phenol red and urea hydrolysis

Answer 3

Phenol red

Question 4

Ph indicator for decarboxylation

Answer 4

Bromcresol purple

Question 5

Ph indicator for MR

Answer 5

Methyl red

Question 6

Ph indicator for citrate and of-glucose

Answer 6

Bronthymol blue

Question 7

Positive for of glucose

Answer 7

Yellow… Can ferment glucose

Question 8

Phenol red positive

Answer 8

Yellow… Can ferment carbs

Question 9

Pink for phenol red

Answer 9

K

Question 10

Positive for nitrate reduction

Answer 10

Red after A and B or clear after zinc

Question 11

Positive for oxidase

Answer 11

Blue TMPD oxidized

Question 12

Positive for decarboxylation

Answer 12

Purple… Can ferment AA and glucose

Question 13

Positive for MR

Answer 13

Red… Mixed acid fermentation

Question 14

Positive for indole

Answer 14

Red ring at top

Indole and ammonia produced

Question 15

VP positive

Answer 15

Red ring at top

Acetoin and 2,3 butanediol fermentation

Question 16

Citrate positive

Answer 16

Blue

Alkaline products

Question 17

Urea positive

Answer 17

Pink

Alkaline products

Question 18

What’s the role of TMPD in oxidase test

Answer 18

CHROMOGENIC reducing agent

Blue bc donated electron (oxidized)

Question 19

Psychrophile

Answer 19

-5 - 20

Question 20

Psychrothroph

Answer 20

0-30

Question 21

Mesophile

Answer 21

15-40

Question 22

Thermophile

Answer 22

40-75

Question 23

Extreme thermopile

Answer 23

65-110

Question 24

Acidophile

Answer 24

Lower than 4.5-5.5

Question 25

Neutrophile

Answer 25

5.5-8

Question 26

Alkalinephile

Answer 26

Higher than 8-8.5

Question 27

Halophile

Answer 27

Greater than 3%

Question 28

Extreme halophile

Answer 28

15%-25%