mass spectrometry Flashcards
chapter 2,4
what is mass spectrometry
technique used to measure the mass of atoms or molecules
what can mass spectrometer determine
relative isotopic mass and isotopic abundance
mass spectra
plots relative abundance of each ion against mass to charge ratios
number of peaks in mass spectra
indicates number of isotopes
position of each peak
indicates relative isotopic mass
heights of peak
relative abundance
1) vaporisation
chosen element is heated and vaporized in a vacuum chamber and passed through mass spectrometer
2) ionization
vapor passes through high energy electron chamber, where collisions with beam causes atoms to lose one of their electrons and form ions
3) acceleration
ions are acceleration by electric field to form a high speed of positive ions that is directed through a strong magnetic field
4) deflection
individual ions become deflected by the field and move into circular paths of different radius depending on mass and charge. Lower mass to charge ratio means greater deflection.
5) detection
detector measures the intensity and radius of deflection and outs it into a mass spectra with isotopic abundance and isotopic mass, mass to charge ratio