mass spectrometry Flashcards

chapter 2,4

1
Q

what is mass spectrometry

A

technique used to measure the mass of atoms or molecules

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2
Q

what can mass spectrometer determine

A

relative isotopic mass and isotopic abundance

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3
Q

mass spectra

A

plots relative abundance of each ion against mass to charge ratios

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4
Q

number of peaks in mass spectra

A

indicates number of isotopes

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5
Q

position of each peak

A

indicates relative isotopic mass

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6
Q

heights of peak

A

relative abundance

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7
Q

1) vaporisation

A

chosen element is heated and vaporized in a vacuum chamber and passed through mass spectrometer

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8
Q

2) ionization

A

vapor passes through high energy electron chamber, where collisions with beam causes atoms to lose one of their electrons and form ions

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9
Q

3) acceleration

A

ions are acceleration by electric field to form a high speed of positive ions that is directed through a strong magnetic field

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10
Q

4) deflection

A

individual ions become deflected by the field and move into circular paths of different radius depending on mass and charge. Lower mass to charge ratio means greater deflection.

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11
Q

5) detection

A

detector measures the intensity and radius of deflection and outs it into a mass spectra with isotopic abundance and isotopic mass, mass to charge ratio

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