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Frank's QA/QC lecture > Mass spectrometry 1 & 2 > Flashcards

Mass spectrometry 1 & 2 Flashcards

1
Q

What are the basic steps in mass spec?

A
  • Generate molecule ion gas phase
  • Ionisation
  • Mass seperation
  • Ion detection
  • Data interpretation
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2
Q

What si the most common form of ionisation?

A

Electron ionisation

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3
Q

What are the cons of EI?

A
  • Hard ionisation
  • Not always a clear molecular ion
  • Fragmentation needed to solve molecular structure of complex molecules
  • Difficult ot identify fragile molecules
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4
Q

What si the base peak?

A

tallest peak

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5
Q

Detection step is sensitive, minimum sample size for analysis. TRUE OR FALSE?

A

TRUE

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6
Q

Isotope disrtibution reveals the amount of Cl,Br, and C. TRUE OR FALSE?

A

TRUE

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7
Q

Isomers do not have the same masses. TRUE OR FALSE?

A

False

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8
Q

Every odd electron is an OE ion. TRUE O FALSE?

A

-TRUE

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9
Q

Rearrangements involving multiple bond breaking can also yield OE ions. TRUE OR FALSE?

A

TRUE

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10
Q

Formation of very samll ions unlikely. TRUE OR FALSE?

A

TRUE

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11
Q

Most stable ion is the largest peak (tertiary is more stable than secondary which is more stable than primary). TRUE OR FALSE?

A

TRUE

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12
Q

If ions are stable in solution, the same applies to gas. TRUE OR FALSE?

A

TRUE

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13
Q

Radicals are less stable with increased substitution. TRUE OR FALSE?

A

FALSE

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14
Q

Vinyl radicals are unstable. TRUE OR FALSE?

A

TRUE

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15
Q

If primary fragmentation leads to competition then loss of larger radical normal dominant. TRUE OR FALSE?

A

TRUE

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16
Q

What are the features for fingerprinting?

A
  • Compare to database of spectra
  • Needs to be established first
  • Spectra specific for specific conditions
  • EI mainly
  • Other MS techniques much smaller databases
  • Make own dedicated database of limited size
17
Q

What is soft ionisation?

A

Only adds charge to molecule for easy identification of molecular ion (molecular mass)
-If you know the precice mas very well you can calculate the number of C,H,O,N,S

18
Q

What does chemical ionisation do?

A

-Controls what functionality is ionised

19
Q

Electrospray ionisation is very good for large biomolecules. TRUE OR FALSE?

A

TRUE

20
Q

What solvents are used in ESI?

A

-MeOH/H20 mixtures

21
Q

What are biomarkers used for?

A
  • To identify biomolecules crucial for disease
  • Undertsand disease more
  • Identify target for treatment
  • Effectiveness of treatment
  • Routine detection of biomarkers using targeted, cheap and fast test

Frank's QA/QC lecture (4 decks)

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