MASS SPEC

Question 1

An instrumentaltechnique used to confirm the molar mass of a substance or molecule through the use of a mass spectrometer

Answer 1

mass spectrometry

Question 2

instrumental analytical technique used to measure the mass-to-charge ratio of fragment ions in order to elucidate the chemical identity and structure of molecules.

Answer 2

mass spectrometry

Question 3

bombards the molecule under investigation with an electron beam and quantitatively records the result as a spectrum of positive ion fragments (mass spectrum)

Answer 3

mass spectrometer

Question 4

It is the transition of the analyte from the solid or liquid phase into the vapor
phase.

Answer 4

Volatilization

Question 5

the first crucial and challenging step in the
analysis of organic compound through mass spectrometry.

Answer 5

Ionization

Question 6

This involves the conversion of neutral molecule to a gas-phase ionic specie with the use of various techniques such as ejection or capture of an electron by an
analyte.

Answer 6

Ionization

Question 7

move randomly and their separation by gravitational force seems highly impractical

Answer 7

neutral gas-phase species

Question 8

the component of the mass spectrometer that takes ionized fragments and separates them based on charge to mass ratios and outputs them to the detector where they are detected and later converted into a digital output.

Answer 8

mass analyzer

Question 9

are used for mass focusing and focus angular dispersions. It separates the ions while on flight using a magnetic field. (electrostatic field before magnetic field)

Answer 9

Magnetic field deflection spectrometer

Question 10

separates ions of different masses by
making use of their different velocities after
acceleration through a potential V

Answer 10

Time of flight spectrometer

Question 11

It separates ions by time without the use of electric or magnetic field. The separation of ions is based on the kinetic energy and velocity of the ions in similar sense as that of chromatography.

Answer 11

Time of flight spectrometer

Question 12

It uses an electric field for the separation of ionic fragments by mass to charge ratios.

Answer 12

Quadrupole spectrometer

Question 13

It uses a magnetic field to trap ions into an orbit inside of it.

Answer 13

Ion cyclotron resonance mass spectrometer

Question 14

there is no occurrence of ion separation rather all the ions of particular range of m/e values are trapped inside and an applied external electric field helps to generate a signal.

Answer 14

Ion cyclotron resonance mass spectrometer

Question 15

This utilizes two or more spectrometers which are coupled together using an additional reaction step to increase their abilities in analyzing chemical samples.

Answer 15

Tandem mass spectrometer

Question 16

The ion is vibrationally excited by collision with the target gas (i.e. Argon) that generates ion fragments that are separated and recorded by a second mass analyzer

Answer 16

Collision-induced dissociation (CID)

Question 17

It is a method of fragmenting gas phase ions for structural elucidation
of peptides and proteins.

Answer 17

Electron capture dissociation (ECD)

Question 18

It is a method of fragmenting multiply-charged gaseous macromolecules in a mass spectrometer between the stages of
tandem mass spectrometry.

Answer 18

Electron transfer dissociation (ETD)

Question 19

It is a technique used in mass spectrometry to fragment molecules in the gas phase for structural analysis of the parent molecule.

Answer 19

Infrared multiproton dissociation (IRMPD)

Question 20

Ions are trapped in an ultrahigh vacuum by a magnetic and an electric field and rapidly exchanged blackbody photons with the vacuum chamber walls in the mass spectrometer.

Answer 20

Blackbody infrared radiative dissociation (BIRD)

Question 21

device for introducing the sample

Answer 21

Sample handling system

Question 22

for determining the
amount of sample introduced

Answer 22

micromanometer

Question 23

a device for metering the sample to the ionization chamber

Answer 23

molecular leak

Question 24

This component is responsible for the fragmentation of molecules before they
are mass analyzed.

Answer 24

Ionization & accelerating chambers

Question 25

a magnetic field is applied to the analyzer tube

Answer 25

ion beam focusing unit

Question 26

This component is responsible for the mass analysis of cationic fragments.

Answer 26

Analyzer tube & magnet

Question 27

This component is responsible
for the detection of cationic fragments.

Answer 27

Ion collector and amplifier

Question 28

produces the signals obtained in mass
spectrometric analysis

Answer 28

Recorder

Question 29

– yields an odd-electron positively
charged cation radical [M]+.
of the same mass as the
initial molecule M

Answer 29

electron ejection

Question 30

This involves the spectral analysis without considering the breaking of chemical bonds.

Answer 30

Soft analysis

Question 31

This analysis considers the breaking of chemical bonds during ionization
of the molecule and thus requires fragmentation analysis from the mass
spectral data.

Answer 31

Hard analysis

Question 32

molecular fingerprint and very unique for a
specific molecule.

Answer 32

fragmentation pattern