Mass spec

Question 1

What is Trypsinization?

Answer 1

Digestion of protein by trypsin

Question 2

What is peptide map fingerprints?

Answer 2

peptides unique for a protein

Question 3

How does mass spectrometer separate ions?

Answer 3

mass/charge ratio

Mass constant, different charges

Single charged, doubly charged, triple charged

Question 4

The degree of deflection depends on what?

Answer 4

Molecular mass of ions (m/z ratio)

Smaller gets deflected more (requires less force)

Bigger gets deflected less

Question 5

What is ESI-MS?

Answer 5

knocks out electrons making protein positively charged

Proteins with the most charge reaches detector first while least charge will arive last (most + AA which are Cys, Thr, Arg)

Question 6

What is MALDI-MS?

Answer 6

treat with acid which will give protons

all +1 charge with different masses

Since all have +1 charge, the m/z ratio will start from the smallest to the largest

Question 7

What is the tallest peak called?

Answer 7

base peak

Question 8

What is the rightmost peak called?

Answer 8

parent peak