Manipulation Of Nucleic Acids

Question 1

Endonucleases

Answer 1

Cut DNA or RNA in the middle

Question 2

Exonucleases

Answer 2

Remove single nucleotides from each end

Question 3

Restriction enzymes

Answer 3

Are endonucleases that cut DNA at specific nucleotide sequences

Question 4

DNase

Answer 4

Deoxyribonuclease is an enzyme that cuts or degrades DNA

Question 5

Modification enzyme

Answer 5

Enzyme that binds to the DNA at the same recognition site as the corresponding restriction enzyme but methylates the DNA

Question 6

Nuclear

Answer 6

Enzyme that cuts or degrades nucleic acids

Question 7

Recognition site

Answer 7

Sequence of bases on DNA that is recognized by a specific protein like restriction enzyme

Question 8

Restriction enzyme

Answer 8

Type of endonuclease that cuts double stranded DNA after binding to a specific sequence of bases, the recognition site

Question 9

RNase

Answer 9

Ribonuclease is an enzyme that cuts or degrades RNA

Question 10

DNA ligase

Answer 10

Enzyme that joins DNA fragments covalently end to end

Question 11

Restriction map

Answer 11

A diagram showing the location of restriction enzyme cut sites on a segment of DNA

Question 12

T4 ligase

Answer 12

Type of DNA ligase from bacteriophage T4 that is capable of ligating blunt ends

Question 13

Blunt ends

Answer 13

Ends of a double stranded DNA molecule that are fully base paired and have no unpaired single stranded overhang

Question 14

Sticky ends

Answer 14

Ends of double stranded DNA that have unpaired single stranded overhangs, generated by a staggered cut

Question 15

Type II restriction enzyme

Answer 15

Restriction enzyme that cuts the DNA in the middle of the recognition site

Question 16

Type I restriction enzyme

Answer 16

Restriction enzyme that cuts the DNA a thousand or more base pairs away from the recognition site