Making and purifying proteins

Question 1

Step 1

Answer 1

1. Retransform bacteria with the engineered plasmids
   Plate on LB/AMP (Luria-Bertani/Ampicillin) plate where bacteria make protein of interest

Question 2

Step 2

Answer 2

Grow up lots of bacteria
Transfer to 1L flasks

Question 3

Step 3

Answer 3

Lyse bacteria (Kill/Explode bacteria) (Sonication)
Collect lysate

Question 4

Step 4

Answer 4

Centrifuge to Separate bacterial debris from proteins
Collect proteins

Question 5

Step 5

Answer 5

Purify the wanted antigen away from other proteins
Column Chromatography
Separate proteins by size
Works like a filter

Question 6

Step 6

Answer 6

We now have our desired antigens from a specific virus, which we can inject into the body (known as Subunit Vaccine).
The immune system then can react to all the separate antigens the same way it would with an actual virus, and create an immune defense against that virus, without having the body contract the disease.
Somewhat similar to Inactivated Virus Vaccines