Maintaining a Balance Flashcards
What are enzymes?
Globular proteins; increase reaction rate (catalyse)
Unchanged at end of reaction
Bind to substrate (active site)
Chemical Composition of Enzymes
Protein molecules (amino acid chain) fold in specific shape
Act on reactant molecule (substrate) fit with at specific location on enzyme molecule surface (ac
Role of Enzymes in Metabolism
Acceleration of chemical reactions
Lowers activation energy needed for reaction; reaction starts quickly without temp change
Lowering of activation energy
Brings specific molecules together (instead of relying on random collisions)
Action on specific substrates
Only one particular enzyme works on one particular substrate molecules
Active site is reciprocally shaped to bind with that molecule
Characteristics of enzymes
Temperature sensitive
Function best at body temp (above 60℃→ stop working)
Heat breaks hydrogen bonds→ alters active site (not reciprocally shaped)
Temp too high or low→ will denature
pH sensitive
Narrow pH range functions efficiently; levels outside optimum; alters shape
Substrate specific
Each enzyme catalyses one particular reaction; act on one substrate
Enzyme Models
Induced fit
Enzyme changes shape as substrate approaches (molecules flexible)
Reaction occurs, substrate changes, product released (enzyme returns to original form)
E.g. Gloved hand changes to catch ball; active site is palm, closes around ball when it draws near
Lock and key
Simply fits into active site to form immediate reaction (not considered accurate)
Depends on unlikely random collisions between enzyme and substrate
E.g. Like trying to get key in lock by throwing key at lock with eyes closed
Identify data sources, plan, choose equipment or resources and perform a first hand investigation to test the effect of:
Increased temperature
Change in pH
INCREASED TEMP
Milk with rennin; curdled quickly (temp approx 370C)
Temps higher or lower than optimum→ milk with rennin doesn’t curdle (doesn’t react)
CHANGE IN pH
pH affects activity of catalase in potato tissue (has optimum pH)
Height of foam measured when catalase put in hydrogen peroxide
pH of 9 is optimum for catalase (average bubble height was higher)
Identify the pH as a way of describing the acidity of a substance
pH scale→ indicates acidity
Lower value; acidic,
Higher value ;alkaline
Explain why the maintenance of a constant internal environment is important for optimal metabolic efficiency
Stable for enzyme functioning→ maintain metabolism (enzymes sensitive to change)
Small variations from narrow range→ small decreases in activity
Larger variation from narrow range → reduced metabolic efficiency
Gather, process and analyse information from secondary sources and use available evidence to develop a model of a feedback mechanism
Stimuli→ increased or decreased body temp (E.g. hot/cold surroundings, exercise)
Co-ordinating centre→ Hypothalamus detects change; activates cooling or warming mechanism
Effectors:
High temp; Skin vessels dilate (blood carries heat to skin surface) Sweat glands (evaporate)
Low temp; Skin vessel constrict (reduce heat loss from skin surface) Skeletal muscles (shiver)
Negative feedback loop→ body temp increases or decreases, hypothalamus shuts off warming or cooling mechanism
Describe homeostasis, as the process by which organisms maintain a relatively stable internal environment
Maintenance of constant (or almost) internal state, regardless of external environmental change
Body regulates respiratory gas, protects against pathogens, maintain salt/fluid balance, constant temp
Regardless of environmental change→ body temp, blood pH, water/salt balance, blood pressure, oxygen, carbon dioxide concentration; kept constant.
Explain that homeostasis consists of 2 stages:
- Detecting changes from the stable state
- Counteracting changes from the stable stage
Any internal deviation must be quickly corrected. Counteract; use corrective mechanism
Stage 1: Detect change from stable state: Receptors detect change. E.g. Thermoreceptors in skin
Stage 2: Counteract change: Effector (muscle or gland) receives message to counteract change. Response initiated to reverse change, restore body to stable. E.g. Muscles shiver to generate heat
If variation exceeds normal; NEGATIVE FEEDBACK counteracts, returns body to homeostasis
Analyse information from secondary sources to describe adaptations and responses that have occurred in Australian organisms to assist temperature regulation
GENERATE HEAT
RETAIN HEAT
GENERATE HEAT
Shivering: Rapid muscle contractions
Increased metabolism; Activity of thyroid gland stimulated, speeds up metabolism
RETAIN HEAT
Raised hair: traps warm air, reduces heat loss by convection. Muscles contract
Vasoconstriction: Blood vessels construct so heat carried in blood is redirected to core of body, prevents heat loss from body surface
Analyse information from secondary sources to describe adaptations and responses that have occurred in Australian organisms to assist temperature regulation
RELEASE HEAT
GENERATE LESS HEAT
RELEASE HEAT
Vasodilation: Arterioles expand, blood directed to body surface, heat lost by radiation, convection
Sweating: liquid secreted onto skin, heat removed to evaporate liquid
GENERATE LESS HEAT
Decreased metabolism: Thyroid gland lowers metabolism, generates less heat
Flattened hairs: Laid flat, increases heat loss
Outline the role of the nervous system in detecting and responding to environmental changes
Function of nervous system→ coordination
Receptors; Thermoreceptors, hypothalamus detects change → converts to message, travels along nerves in CNS (brain, spinal cord)
Control centre: CNS processes info about change in specific parts of brain
Motor nerves; Carry info as nerve impulses from CNS to effectors
Effectors: Muscle or gland receives impulses, instruct effectors to respond
Response; Counteracts original change; ensures homeostasis
Identify the broad range of temperatures over which life is found compared with the narrow limits for individual species
Living creatures can survive temps of -70℃ (poles), high as 56℃ (deserts), 350℃ ( hot vents in sea)
Individual species need much narrower range of temp (have optimum temp they function at)
Tolerance range; temp range species can survive, usually few degrees outside of optimum
Endothermic definition
Endothermic; maintain constant internal temp; using internal metabolism to generate heat (mammals)
Ectothermic definition
Ectothermic: Body temp governed by external heat sources, environment regulates temp (reptiles)
Endothermic example
Red Kangaroo
Hottest part of day→ seek shade; tail, hind legs shade by body (reduces surface are exposed to sun)
Lowers body temp
Ectothermic example
Blue tongue Lizard
Cold weather→ remain inactive (buried in shelter) lowers metabolic rate→ conserve energy
Sunny days→ emerge to bask→ raises temp
Some responses of plants to temp change
LEAF FALL
SHINY LEAVES
ORIENTATION
ICE FORMATION BETWEEN CELLS
Leaf fall
Hot conditions→ plants drop leaves (reduces surface area to sun, reduces water loss through transpiration)
Shiny leaves
Reflect solar radiation→ reduces heat absorbed
Orientation
Vertical orientation→ reduces surface area to sun, reduces amount of heat exposed to)
Ice formation between cells
Temps below freezing→ ice form in cells, forms in gaps between plant cells; cell walls protects cytoplasm being pierced by ice crystal→ cell survives
PLANTS AND ANIMALS TRANSPORT DISSOLVED NUTRIENTS AND GASES IN A FLUID MEDIUM
Transport system; distributes food/oxygen to cells, removes carbon dioxide and waste
Blood; fluid transport medium; contains 3 types of cells
RBC: Carry oxygen, maintain pH of blood
WBC: Part of immune system, protects against invading organism
Platelets: Clotting of blood, stops blood loss
Plasma; Makes up most blood volume; carries nutrients, gases etc
Identify the form(s) in which each of the following is carried in mammalian blood:
OXYGEN
Carried from lungs to heart, body tissues
98.5% as hemoglobin in RBC, 1.5% dissolved in plasma
Identify the form(s) in which each of the following is carried in mammalian blood:
CARBON DIOXIDE
Cellular respiration product carried to lungs
70% as hydrogen carbonate ions, 7% as plasma, 23% combined with haemoglobin
Travels in RBC, plasma
Identify the form(s) in which each of the following is carried in mammalian blood:
WATER
Reabsorbed from nephron to body cells
Travels in plasma as water molecules
Identify the form(s) in which each of the following is carried in mammalian blood:
SALTS
Reabsorbed from nephrons to all body cells.
Dissolved in plasma as ions
Identify the form(s) in which each of the following is carried in mammalian blood:
LIPIDS
Absorbed across villi wall of small intestine to veins in shoulder,
As fatty acids, glycerol dissolved in plasma
Identify the form(s) in which each of the following is carried in mammalian blood:
OTHER DIGESTION PRODUCTS (amino acids, glucose)
Proteins broken down into amino acids, transported
across small intestine wall.
Dissolved in plasma to be absorbed into cells for making proteins
Identify the form(s) in which each of the following is carried in mammalian blood:
NITROGENOUS WASTES
Urea processed in liver → moves into blood
Transported dissolved in plasma to kidneys (removed across nephrons)
Perform a first- hand investigation to demonstrate the effect of dissolved carbon dioxide on the pH of water
Water in beaker (add universal indicator)
Blow bubbles with straw (carbon dioxide) for 2 mins
Colour will change→ estimate pH using colour chart (makes more acidic)
Perform a first hand investigation using the light microscope and prepared slides to gather information to estimate the size of red and white blood cells and draw scaled diagrams of each
Known diameter of RBC= 7.5um
Calculate field of view (mini grid) → on slide estimate number of RBC that fit across diameter of fov
Estimate number of WBC, repeat and compare with known
What is Haemoglobin?
Oxygen carrying molecule (carries 4 oxygen molecules)
Each RBC carried 200-300 million haemoglobin molecules→ so 800-1200 million oxygen molecules
Protein of 4 polypeptide chains (globins) bonded to iron containing group (haem)
Adaptive advantage of Haemoglobin:
Increases oxygen-carrying capacity of blood
1 haemoglobin molecule binds with 4 oxygen molecules
More oxygen can be carried in blood cells
Adaptive advantage of Haemoglobin:
Increases binding of oxygen once first oxygen molecule binds
Bonding causes haemoglobin to change slightly, easier for subsequent oxygen molecules to bind
Increases rate and efficiency of oxygen intake
Adaptive advantage of Haemoglobin: Release of oxygen increases when carbon dioxide is present
Has to release oxygen from blood to where it’s needed
Metabolising cells release carbon dioxide (lowers pH)
Haemoglobin at lower pH has lowered attraction to oxygen (can release)