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MICROBIOLOGY > ma'am Aldave handout > Flashcards

ma'am Aldave handout Flashcards

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Biology 101 flashcards
1
Q

x and v factors

A

h. influenza

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2
Q

v factor (NAD)

A

H. parainfluenzae

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3
Q

o.5% Mcfarland

A

adjust turbidity

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4
Q

colorless on XLD

A

Shigella

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5
Q

coagulase

A

confirm S.aureus

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6
Q

Endospore/bamboo arrangement

A

seen in cultures

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7
Q

autoclave, UV, filter, oxide

A

sterilize decontaminate materials

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8
Q

oxk ox19

A

weil felix test

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9
Q

confirm leptospirosis

A

culture

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10
Q

gray colonies

A

c.diphtheriae

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11
Q

milk borne dx

A

s. typhis?/s.bovis

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12
Q

CSF media

A

BAP and CAP

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13
Q

QC on oxidase and Catalase

A

every day and once opened

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14
Q

Capsule- sero typing

A

strep, pneumoniae (quellung rxn)

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15
Q

g(+) spore, drum stick

A

C. tetani

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16
Q

kidney bean shape

A

neisseria

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17
Q

fluorescent stain

A

for darkfield microscope

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18
Q

blue green/ fruity odor

A

P. aeruginosa

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19
Q

rotavirus, ETEC

A

common diarrhea world wide (c. jejuni); infantile diarrhea

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20
Q

hand drier

A

no contamination

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21
Q

ECHO

A

enteric cytopathic human orphan

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22
Q

foot mouth hand dx

A

conxsackie A

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23
Q

drug resistance from MICROORGANISM

A

Biological resistance

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24
Q

Anaerobic atmosphere

A

5% CO2, 10% H2, 85% N2 (Gas pak jar)

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25
Q

Microaerophillic/Campy gas

A

5% O2, 10% Co2, 85% N2

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26
Q

Drug resistance from PATIENTS

A

Clinical Resistance

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27
Q

Not automated machine in micro

A

Vitek, Bactec 9240, bact/alert, advanced expert system, PCR

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28
Q

iodophor

A

iodine and quats

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29
Q

BSC class II

A

exhausted and recirculated air

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30
Q

differentiation of GS

A

decolorization

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31
Q

AFO

A

pink

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32
Q

acridine orange

A

nucleic acid

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33
Q

Throat swab for anaerobic culture

A

reject

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34
Q

Throat pathogen

A

S. pyogenes

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35
Q

CNA agar

A

g(+)cocci

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36
Q

adult viral gastroenteritis

A

Norwalk

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37
Q

Irregular, serrated, swirling projection, gas gangrene (double hemolysis??)

A

B. anthracis

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38
Q

Serum tube test

A

C. albicans

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39
Q

Czapeks agar

A

Aspergillus

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40
Q

Citrate acetate color

A

blue

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41
Q

PYR-, Bacitracin(r), camp (+ )

A

s.agalactiae

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42
Q

nitrate test with zinc dust no change

A

true positive

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43
Q

needlestick/brokenglass

A

direct inoculation

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44
Q

confirmed water test

A

EMB, ENDO, BGBL

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45
Q

NAFO colour

A

blue

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46
Q

CSF storage

A

37c

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47
Q

optochin

A

Taxo P, ethylhydrocupreine HCL

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48
Q

6.5% NAcl, Hippurate, Esculin (+)

A

Entercoccus

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49
Q

held on colony or filter paper

A

Oxidase test

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50
Q

MUG(+) coliforms

A

yellow (colorimetric assay) b-glucoronidase

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51
Q

PPNG

A

Penicillinase producing gonococci

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52
Q

critical in GS

A

decolorization step

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53
Q

betalactamase test

A

colorimetric method(+) colour change

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54
Q

Schaudinn stain

A

mitochondria (internal) parasite

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55
Q

largest RNA virus

A

Paramyxo

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56
Q

biochemical test

A

presumptive test

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57
Q

beta lactamase substrate

A

nitrocefin

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58
Q

AFB culture

A

Urine, CSF, stool, gastric

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59
Q

CIN

A

Cefsulodin Irgasan Novobiocin Y. Enterolitica

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60
Q

confirm typhoid fever

A

bone marrow culture

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61
Q

presumptive ID of organism

A

differential

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62
Q

CMV culture

A

human embryonic fibroblast

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63
Q

h20 completed test

A

acid gas

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64
Q

acetamide by product

A

ammonia

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65
Q

fungal culture (CSF)

A

28-30 days

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66
Q

spirochetes microscope

A

fluorescent

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67
Q

household bleach

A

10 minutes

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68
Q

bench marking

A

peer comparison

69
Q

optochin for s.pneumoniae

A

> 14mm zone

70
Q

double zone of hemolysis

A

c. perfringens

71
Q

hemagglutination inhibition

A

influenza Rubella

72
Q

difficult to stain

A

leptospira

73
Q

normal oral flora

A

viridans

74
Q

s.aureus on stool

A

MSA (7.5% Nacl)

75
Q

plasma coagulase

A

rabbit human

76
Q

enhancement media

A

BAP, Thio, selenite

77
Q

+bile solubility

A

lysis of colony

78
Q

species written

A

lower case

79
Q

babes ernst

A

c.diptheriae

80
Q

iodine and bleach

A

iodophore

81
Q

lecinthinase (+)

A

C.perfringens

82
Q

Blood culture disinfection

A

iodine and 70% alcohol

83
Q

veilonella and actinomyces

A

anaerobic

84
Q

completed water bacte (+)

A

gas and growth

85
Q

Most Probable Number MPN

A

water bacte

86
Q

dissolve agar

A

half h20 +half agar + half h20

87
Q

Duran reynal factor

A

spreading factor

88
Q

Ag, lytic, anaerobic, O2 labile

A

streptolysin O

89
Q

DOH 2013 sputum AFB

A

2

90
Q

coagulase test

A

rabbit human plasm

91
Q

ratio of Blood:broth

A

10ml:50ml

92
Q

anti fungal, anti gram (+) MAC

A

crystal violet

93
Q

kidney bean shaped

A

Neisseria

94
Q

curve gram - rod medium

A

CBAP

95
Q

+ hair baiting test

A

v shape formation

96
Q

farmer’s lung dx

A

aspergillus

97
Q

CNS virus causing Encephalitis

A

Rabies virus

98
Q

HTLV I and II specimen

A

blood, serum, saliva, semen

99
Q

transport virus for days.

A

-70 c

100
Q

photometry

A

104 or 105 CFU/ml

101
Q

before and after pasteurization

A

75-15k

102
Q

AGN and RF

A

group A strep

103
Q

Asteroid body

A

production of Ag-Ab rxn

104
Q

Streptolysin O

A

O2 labile, Ag, Anaerobic lytic positive

105
Q

E coli MR/VP

A

red

106
Q

transport temp of virus and sputum

A

4c

107
Q

type of water for agar

A

Deionized/distilled

108
Q

HEPA filter

A

0.3 microns

109
Q

photoreactivity

A

photochromogens

110
Q

kills m.tb

A

boiling

111
Q

anaerobic sampling

A

70% ethyl alcohol

112
Q

peptic ulcer/ cancer

A

H. pylori

113
Q

measure susceptibility

A

Caliper/ruler

114
Q

automation for susceptibility

A

Vitek

115
Q

N. gonorrhea

A

eyes, vaginal discharge, gastric fluid

116
Q

C. albicans in serum

A

germ tube

117
Q

M.Tb clear media

A

middlebrook 7H10/11

118
Q
        • mucoid colonies
A

klebsiella

119
Q

gas gangrene

A

C. perfringens

120
Q

Hep. C test of choice

A

HCV RNA

121
Q

complete virus particle

A

virion

122
Q

catalase(+) coag (-) non hemo.

A

S.epidermidis

123
Q

bacteria same size as virus

A

mycoplasma

124
Q

bacteria from cows causing colon cancer

A

S. bovis

125
Q

green pus fruity odor, serrated edges

A

p.aeruginosa

126
Q

flat irregular edges, swirling projections non hemolytic dry

A

B. anthracis

127
Q

Aman stain

A

Cotton blue

128
Q

CMV

A

human embryonic fibroblast

129
Q

pneumonitis in AIDS patients

A

P.carinii/jirovecii

130
Q

bacteria thrives best in Cold temperature

A

L. monocytogenes

131
Q

most comonly used for sensitivity

A

Vitek

132
Q

DNASE s. aureus and s.epidermidis

A

S. aureus (+) s. epidermidis (-)

133
Q

virus from male drug user in US

A

HGV

134
Q

commonly isolated organism in ICU

A

P. aeruginosa

135
Q

C. albicans on corn meal agar

A

chlamydospore

136
Q

differential for corynebacteria and listeria

A

motily, esculin

137
Q

toxic to virus

A

calcium alginate

138
Q

largest virus

A

pox virus

139
Q

alternate of darkfield

A

fluorescent microscope

140
Q

guillain baire syndrome

A

Campylobacter jejuni and Chlamydia pneumoniae

141
Q

Acetamide

A

35c up to 7 days

142
Q

bacitracin (+) control

A

S. pyogenes

143
Q

MUG negative

A

no blue fluorescence

144
Q

P. fluorescens

A

bacteremia

145
Q

Bacteria has dna and Rna

A

virus either

146
Q

collect blood

A

before the height of fever

147
Q

rickettsia stain

A

Gimenez , Macchiavelo

148
Q

Anton agent

A

listeria

149
Q

XLD yellow

A

E.coli

150
Q

lockjaw

A

C. tetani

151
Q

malonate -

A

green/yellow

152
Q

ph of culture media for bacteria

A

ph 7.2-7.4

153
Q

diptheria toxin in vitro

A

Elek test

154
Q

lowest conc of drug to kill organism

A

MBC or MLC

155
Q

differentiate S. pyogenes and S. pneumoniae

A

hemolysis test

156
Q

differentiate listeria and corynebacteria

A

esculin hydrolysis

157
Q

penicillin resistant

A

beta lactamase

158
Q

endospore stain

A

schaeffer fulton

159
Q

diplococci ear infection

A

Moraxella/Branhamella

160
Q

arthrobacter

A

brevibacterium

161
Q

unique in cell wall of AFB

A

mycolic acid

162
Q

curved bacteria w/ darting motility

A

campylobacter

163
Q

filamentous bacteria, antibiotics

A

streptomyces

164
Q

dermatophytoses

A

skin, hair, nail

165
Q

effect of too much moisture in disk diffusion

A

smaller zone

166
Q

salmonella in SS agar

A

colorless colonies w/ black centers

167
Q

best for salmonella culture

A

SS, XLD, tetrathionate

168
Q

sterilization kills

A

C. botulinum, endospores of e.coli

MICROBIOLOGY (2 decks)

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