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BioChem Finals > M7 > Flashcards

M7 Flashcards

1
Q

____ and ____ are formed when amino acids are joined together by amide bonds

A

Peptides and Proteins

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2
Q

___ is called also peptide bond

A

Amide bond

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3
Q

___ has 2 amino acids joined together by one peptide bond

A

Dipeptide

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4
Q

___ have many amino acids

A

Polypeptide

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5
Q

___ has more than 40 amino acids

A

Protein

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6
Q

_____ are formed by a condensation reaction

A

Peptide bonds

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7
Q

___ are joined together and water is released

A

Amino Acids

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8
Q

What is the formation of polypeptides?

A
  1. Remove oxygen from the precedent amino acid and two hydrogens from the subsequent amino acid
  2. Place the two groups next to each other
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9
Q

General test for identification of proteins

A

Biuret test

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10
Q

In the presence of _____ _____, Cu2+ ions forms as a complex with the peptide bonds due to the unshared electron pairs in nitrogen, and the oxygen in water

A

Alkaline Solution

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11
Q

Biuret Test: Gelatin
Result: ?

A

Purple Solution

Proteins (peptide bonds) are present

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12
Q

Biuret Test: Egg Albumin
Result: ?

A

Purple Solution

Proteins (peptide bonds) are present

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13
Q

Biuret Test: Glycine
Result: ?

A

No Reaction

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14
Q

Biuret Test: Antibiotic Solution
Result: ?

A

Pink Solution

Proteins (peptide bonds) are present

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15
Q

Test for the presence of a-amino acids in proteins

A

Ninhydrin Test

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16
Q

___ causes oxidative decarboxylation and deamination of a-amino acids producing an aldehyde, carbon dioxide, and ammonia

A

Ninhydrin

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17
Q

Ninhydrin is reduced to _____ which reacts with the liberated ammonia and another molecule of ninhydrin

A

Hydrindantin

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18
Q

Ninhydrin Test: Egg Albumin
Result: ?

A

Deep Blue Solution

Presence of alpha-amino acid

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19
Q

Ninhydrin Test: Gelatin
Result: ?

A

No Reaction

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20
Q

Ninhydrin Test: Alanine
Result: ?

A

Deep Blue Solution

Presence of alpha-amino acid

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21
Q

Test for the presence of aromatic amino acids

A

Xanthoproteic Test

22
Q

Amino acids containtin aromatic nucleus react with concentrated HNO3 to from _____-_____ complex on heating.

It changed to ____-____ _____ when excess NaOH is added

A

Yellow-coloured Complex;

Orange-Red Colour

23
Q

Xanthroproteic Test: Egg Albumin
Result: ?

A

Dark Yellow Solution

Presence of arom

24
Q

Xanthroproteic Test: Tyrosine
Result: ?

A

Orange Red Solution

25
Q

Xanthroproteic Test: Alanine
Result: ?

A

No Reaction

26
Q

Xanthroproteic Test: Phenylalanine
Result: ?

A

No Reaction

27
Q

Test for phenolic compounds

A

Millons Test

28
Q

Millons test is also used to setect the presence of ______ - the only amino acid containing a phrnol group

A

Tyrosine

29
Q

Tyrosine is the first nitrated by nitric acid in the test solution, then the nitrated tyrosine complexes ______ and ______ in the solution to form either a ______ or _____

A

Mercury (I) and Mercury (II)

Red Precipitated or Red Solution

30
Q

Millon’s Test: Phenol
Result: ?

A

No Reaction

31
Q

Millon’s Test: Phenylalanine
Result: ?

A

No Reaction

32
Q

Millon’s Test: Tyrosine
Result: ?

A

Faint Red Precipitate

33
Q

Millon’s Test: Casein
Result: ?

A

No Reaction

34
Q

Test for the presence of tryptophan

A

Hopkin’s Cole Test

35
Q

The inly amino acid containing an indole group

A

Tryptophan

36
Q

The indole reacts with ____ ____ in the presence of a strong acid to form a _____ ______ product

A

Glyoxylic Acid

Violet Cyclic product

37
Q

Hopkins Cole Test: Albumin
Result: ?

A

Violet Ring

38
Q

Hopkins Cole Test: Casein
Result: ?

A

No Reaction

39
Q

Hopkins Cole Test: Gelatin
Result: ?

A

No Reaction

40
Q

Hopkins Cole Test: Tryptophan
Result: ?

A

Violet Ring

41
Q

Test for the presence of cysteine or cystine

A

Lead Sulfide Test

42
Q

The organic sulfur in cystein or cystine is released as ______ - ____ which from lead sulfide

A

Inorganic S-8 ions

43
Q

Positive result of Lead Sulfide Test is the formation of ____ / ____ _______

A

Black or Brown Precipitate

44
Q

Lead Sulfide Test: Albumin
Result: ?

A

Black Solution

45
Q

Lead Sulfide Test: Tyrosine
Result: ?

A

No Reaction

46
Q

Lead Sulfide Test: Casein
Result: ?

A

No Reaction

47
Q

Positive result of Biuret Test

A

Purple / Pink Solution

48
Q

Positive Result of Ninhyrin Test

A

Deep Blue Solution

49
Q

Positive result of Xanthroproteic Test

A

Dark-Yellow / Orange-Red Solution

50
Q

Positive result of Millon’s Test

A

Red Precipitate or Red Solution

51
Q

Positive Result of Hopkins Cole Test

A

Violet Ring

52
Q

Positive Result of Lead Sulfide Test

A

Black / Brown Solution

BioChem Finals (3 decks)

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