M6 Flashcards
most common form of DNA testing,
used to determine the biological relations between individuals.
DNA paternity tests
Dna fingerprinting
(also called DNA typing)
____ can provide positive
identification with great accuracy.
DNA fingerprinting
Two single strands of DNA will undergo digestion by
restriction enzymes to be cut into several fragments
->
1 cm long or 1 micron short
____ are endonucleases which catalyze the
cleavage of the phosphate bonds within both strands of
DNA.
Restriction enzymes
They require ____ (cofactor) for activity and
generate a ___and 3 prime
hydroxyl group at the point of cleavage.
Mg2+, 5 prime phosphate
Distinguished feature: They only cut at very
specific sequences of bases called___
recognition
sites.
Over ___restriction enzymes have been discovered
and catalogued
3,000
Namin enzymes: This is done by using the___
followed by the ____
first letter of the genus,first two letters of the species.
Most recognition sites
are ___base pairs in length.
4 to 8
It is estimated about 25% of all human genes occur in
multiple alleles which are called____
polymorphisms.
constitute a large function of
the mammalian genome and have no known genetic
function
Repetition sequences
Variations in the length of fragments between
different individuals.
● Restriction Fragment Length Polymorphism
(RFLP
Detect a single segment of the repetitive DNA
located at a specific site on a single chromosome
resulting in one or two DNA bands corresponding to
one or both chromosome segments
Single-locus probes (SLPs)
e-locus probes (MLPs)
● Detect multiple repetitive DNA segments located on
many chromosomes yielding 20-30 bands
Multiple-locus probes (MLPs)
