M1: V Flashcards
True or False
The revolving power of a microscope depends mainly on the quality of its objective lens.
True
True or False
The image formed by a compound microscope is real, upright and magnified.
False
True or False
Revolving Turret is a part of the microscope that holds two or more objective lenses and can be rotated to easily change power
True
True or False
The diaphragm collects and focuses a cone of light that illuminates the tissue slide in the stage.
False
True or False
Confocal microscopy involves scanning the specimen at successive focal planes with a focused light beam, often from a laser, and produces a 3D reconstruction from the images.
True
True or False
The eyepiece lens enlarges the image obtained by the objective and improves its resolution.
False
True or False
The maximal resolving power if the light microscope is approximately 0.5nm, a power that permits good images magnified 1000-1500 times.
False
True or False
In a confocal microscope, light from a laser source hits the specimen and is reflected. A beam splitter directs the reflected light to a pinhole and a detector.
True
When using the high power objective, you should not just adjust the:
- Coarse adjustment knob
- Stage clips
- Diaphragm
- Fine adjustment
Coarse Adjustment Knob
What will be the total magnification if the ocular lens is 10x and the objective lens is 40x?
- 40x
- 100x
- 4000x
- 400x
400x
True or False
Polarizing light microscopy produces an image only of material having repetitive, periodic macromolecular structure; features without such structure are not seen.
True
Fill in blank/s
To focus a specimen, it is best to start with which objective:
Scanning
True or False
The wavelength in the electron beam in an electron microscope is much longer than that of light, allowing a 1000-fold increase in resolution.
False
