M1. Lipids_SOLUBILITY TEST

Question 1

what is the objective of SOLUBILITY TEST?

Answer 1

to TEST the SOLUBILITY of OILS in DIFFERENT SOLVENTS.

Question 2

FATS do not dissolved in water because they are?

Answer 2

NON-POLAR/HYDROPHOBIC

Question 3

True or Flase?

FATS are SOLUBLE in ORGANIC SOLVENTS.

such as?

Answer 3

TRUE

benzene
chloroform
boiling alcohol

Question 4

True or False?

Different lipids have the ability to dissolve in different organic solvent.

Answer 4

TRUE

Question 5

Phosphatide lipid cannot dissolve in?

Answer 5

acetone

Question 6

Cerebroside and sphingomyelin cannot dissolve in?

Answer 6

ether

Question 7

Materials used in Solubility Test?

Answer 7

1. OLIVE & COCONUT OIL.
2. Test Tubes.
3. Solvents:
   - Chloroform
   - Ethanol
   - Carbon Tetrachloride [CCl4]
   - Distilled Water [H2O]
   - Sodium Hydroxide [NaOH]

Question 8

STEP 1:

How many mL of SOLVENTS do we need?

Answer 8

1 mL each.

Question 9

STEP 2:

How many DROPS of OILS do we need?

Olive & Coconut oil.

Answer 9

2 drops.

Question 10

after shaking the oils and solvents:

1. if it separated into two layers, the oil is?
2. oil is dissolved in the solvent if ____ is formed.

Answer 10

1. not dissolved
2. one homogenous transparent layer

Question 11

Results:

Chloroform:
1. Olive Oil:
2. Coconut Oil:

Answer 11

1. miscible/soluble
2. miscible/soluble

Question 12

Results:

[Cold] Ethanol:
1. Olive Oil:
2. Coconut Oil:

Answer 12

1. immiscible/insoluble
2. immiscible/insoluble

Question 13

Results:

Carbon Tetrachloride:
1. Olive Oil:
2. Coconut Oil:

Answer 13

1. miscible/soluble
2. miscible/soluble

Question 14

Results:

Distilled Water:
1. Olive Oil:
2. Coconut Oil:

Answer 14

1. immiscible/insoluble
2. immiscible/insoluble

Question 15

Results:

Sodium Hydroxide:
1. Olive Oil:
2. Coconut Oil:

Answer 15

1. immiscible/insoluble
2. immiscible/insoluble