LTM

Question 1

First enzyme discovered: AMYLASE (before: diatase)

Answer 1

Anselme Payen and Jean-Francois Persoz

Question 2

• A proponent of vitalism
• proposed that living yeast cells possess so-called “ferments”,

Answer 2

Louis Pasteur

Question 3

was able to observe the fermentation of
sugar into alcohol without using living yeast cells.

Answer 3

Eduard Buchner

Question 4

coined the term “enzyme”

Answer 4

Friedrich Kuhne

Question 5

• was able to purify and crystallize urease, so it’s another enzyme
• All enzymes are proteins

Answer 5

James Sumner

Question 6

● Discovered ribozymes
● They were working with the mechanism of splicing (the post-transcriptional processing of RNA)

Answer 6

Sidney Altman and Thomas R. Cech

Question 7

Which of the following is FALSE about the Steady State Theory?

a. It is the basis of Menten and Michaelis postulates
b. It has a pre-ready state wherein it is a process in the initial stage that takes place very fast, often in the
microsecond scale.
c. There will be build-up of the ES complex until the steady
state is reached.
d. All of the statements above are true

Answer 7

All of the statements above are true

Question 8

different km, same vmax = slope change

Answer 8

competitive inhibition

Question 9

different km & vmax = slope dont change

Answer 9

uncompetitive inhibition

Question 10

different km & vmax = slope change

Answer 10

mixed inhibition

Question 11

16-carbon SATURATED fatty acid (all single bond)

Answer 11

palmitate

Question 12

18-carbon with 1 unsaturated fat (double bond)

Answer 12

oleate

Question 13

18-carbon with 2 unsaturated fat (double bond)

Answer 13

linoleate

Question 14

18-carbon with 3 unsaturated fat (double bond)

Answer 14

linolenate

Question 15

Brings cholesterol and other lipids inside the cell for utilization (Bad cholesterol)

Answer 15

LOW-DENSITY LIPOPROTEINS (LDL)

Question 16

• Remove cholesterol from cells for excretion (Good cholesterol);
• Carry cholesterol to liver for recycling

Answer 16

HIGH-DENSITY LIPOPROTEINS (HDL)

Question 17

5 inhibitors of etc

Answer 17

cyanide
azide
rotenone
sodium amytal
carbon monoxide

Question 18

lipid-soluble substance that can carry protons across the inner mitochondrial membrane sa etc;

heat generation thru uncoupling

Answer 18

2,4-DINITROPHENOL (DNP)

Question 19

______ are used so that NADH can be used to make ATP

Answer 19

shuttles

Question 20

● Treatment for gout
● Inhibitor of xanthine oxidase
○ Prevents the conversion of uric acid, and just remains as xanthine

Answer 20

ALLOPURINOL

Question 21

• a.k.a the Hexose Monophosphate Shunt
• ONLY source of electron carriers that drive our anabolic pathways (source of NADPH)
• alternative pathway to glycolysis for utilizing glucose

Answer 21

PENTOSE PHOSPHATE PATHWAY

Question 22

____ is stimulated by AMP;
____ is inhibited by AMP; prevents pagsabay ng glycolysis & gluconeogenesis

Answer 22

Phosphofructokinase (stimulated)
Fructose 1,6-bisphosphatase (inhibited)

Question 23

○ “Stress hormone”
○ Controls protein and carbohydrate metabolism
○ Important in the body’s response to
physiologic stress

Answer 23

Cortisol

Question 24

○ Aids in the maintenance of body fluid and electrolyte balance
○ Promotes sodium reabsorption (salt balance) and potassium excretion in kidneys

Answer 24

Aldosterone

Question 25

Male sex hormone
○ Helps in growth and sexual development
■ Precursor of estrogen

Answer 25

Testosterone

Question 26

chemical reaction that that transfers an
amino group to a ketoacid to form new amino acids

Answer 26

transamination