Lp 3 Flashcards

1
Q

Mobilitate bacterii

A

Monotriche si lofotriche rostogolire
Peritriche oscilatorii
Misc browniana a unor particule

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2
Q

Bacterie cu capsula

A

Cryptococcus neoformans

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3
Q

Definitie frotiu

A

Material microbian etalat in strat subțire pe lama marcata cu coloranți pentru examenul microscopic

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4
Q

Timpii de efectuare a unui frotiu

A

Etalare
Uscare rapida
Fixare
Colorare
Protejare cu balsam de canada

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5
Q

Colorația gram etape

A

Col cu violet de gentiana(cristal violet) 1 min
Spălare
Mordantare cu sol Lugol (i2 in ki) 1 min
Spălare
Decolorare cu sol alcool acetona 30sec-1 min
Spalare
Recolorare cu safranina(fucsina bazica) 1 min
Spalare

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6
Q

Structura perete celular gram pozitiv

A

40 straturi de peptidoglican
Acizi teichoici: de perete, de membrana, teichuronici

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7
Q

Structura perete celular gram negativ

A
  1. Peptigoglican 1-2 fire
  2. Strat lipoproteic
  3. Membrana externa(fosfolipide si proteine)
  4. Lipopolizaharidul(lipid a si core)
    5.spatiu periplasmatic( între membrana ext si membrana citoplasmatica)
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8
Q

Culoare gram pozitiv si gram negativ

A

Negativ rosu
Pozitiv albastru-mov

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9
Q

Dc gram negativ rosu

A

Cristalul violet din violetul de gentiana se spala cu sol acid-alcool pentru ca nu adera strâns de perete, pentru ca conține un strat subțire de peptidoglican

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10
Q

Exemplu de bacil gram pozitiv

A

Clostridium tetani

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11
Q

Ex de bacil gram negativ

A

Klebsiella

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12
Q

Coc gram pozitiv

A

Staphilococcus sp.
Strptococcus sp.

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13
Q

Coc gram negativ

A

Neisseria gonohree

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14
Q

Structuri facultative

A

Capsula, glicocalix, pili comuni, pili de sex, cili, spori

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