Liver Function Tests Flashcards
(197 cards)
The chief metabolic organ
liver
Large organ divided unequally into 2 lobes by a ____
falciform ligament
functional unit of the liver
Lobules
2 major type of cells in liver
Hepatocytes
Kupffer cells
AKA hepatic macrophage
Kupffer cells
Produces majority of lobules
Hepatocytes
2 blood supply of liver
Hepatic artery
Portal vein
Supplies 25% of blood
hepatic artery
Supplies majority of blood (75%)
portal vein
blood produced by liver (____ mL/min)
1500
synthetic function secretes ____ (6)
plasma proteins, CHO, lipids, lipoproteins, ketone bodies, & enzymes
Its tests quantitates the severity of hepatic dysfunction
synthetic function
Assessment of nutritional status and presence of severe disease involving the liver, kidney and bone marrow
TP
sample of TP
serum
serum is preferred as sample for TP because plasma contains _____
fibrinogen and anticoagulant
Effect of fibrinogen to TP
increases total protein to 0.2-0.4 g/dL
Effect of anticoagulant to TP
decreases total protein due to dilutional effect
interference of TP
hemolysis, ictericia
serous fluid transudates
<3.0 g/dL
serous fluid exudates
> 3.0 g/dL
methods for TP
Kjeldahl Method
Biuret Method
Folin-Ciocalteu (Lowry) Method
UV Absorption Method
Refractometry
Turbidimetry and Nephelometry
Salt Fractionation
Coomasie Brilliant Blue Dye
Ninhydrin
Serum Protein Electrophoresis
In SPE, Width of the protein band in a particular fraction depends on the ______ in the fraction
concentration of protein
The acidic and basic amino acids content of proteins determines its net charge.
Isoelectric Property of Proteins
pH of SPE
8.6