Listeria monocytogenes Flashcards
how many of the 17 species in the Listeria genus are pathogenic
2: Listeria monocytogenes, Listeria ivanovii (pathogenic in cows)
characteristics of Lm?
- gram positive
- psychrotrophic
- facultative anaerobic
- nonsporulating
- motile
- small rod
- displays motility via its flagella; temperature dependent, motile between 20-30C
what genetic techniques were once used to characterize Lm? what is the main technique now?
genetic finger printing or serotyping
the main technique used today is whole genome sequencing
how many serotypes of Lm are there?
13
what have the 13 serotypes of Lm been characterized into?
4 evolutionary lineages and 63 clonal complexes (CCs)
how old are Lm’s sub-lineages? what did this observation lead to?
50-150 years. observation lead to a hypothesis that Lm may have emerged as a pathogen relatively recently potentially in association with the increased movement of humans, animals, and food
how does Lm live in the environment?
lives ubiquitously in the environment as a saprophyte. it can survive for long periods in soil, food plants, and water
besides animals and humans, what has Lm also been found to infect?
protozoa
true or false: some humans carry Lm without symptoms
true, either in the intestine or the gall bladder
what temperatures can Lm grow and divide at?
4-45C; it grows in the refrigerator and outcompetes other bacteria in this environment
true or false: Lm can grow in a pH as low as 4.4
true
true or false: Lm can grow in high salt concentrations as high as 10%
true; and heat resistance increases as salt content decreases
what is Lm sensitive to?
pasteurization
when can Lm survive pasteurization?
when its in the white blood cell
under what conditions can Lm survive in, on environments like hot dogs, bologna, and ham?
lower temperatures and high salt conditions