Linkage Analysis-Mastick

Question 1

To do linkage analysis, what are the 2 things you need to do?

Answer 1

genotype lots of family memebers

Haplotype pairs of alleles inherited together

Question 2

What are used to detect polymorphisms?

Answer 2

RFLP, VNTR, Microsatellites, SNP (most used)

Question 3

What kinds of tests are these?

• DNA microarrays,
• allele specific oligonucleotides (ASO),
• single-base extension sequencing,

Answer 3

SNP tests

Question 4

What do you have to do with RFLP and VNTR tests?

Answer 4

utilized southern blots and PCR

Question 5

Microsatellite tests use what?

Answer 5

PCR

Question 6

Is hunington and Neurofibrosis 1 dominant or recessive?

Answer 6

dominant

Question 7

Is PKU and Neurofibrosis 1 dominant or recessive?

Answer 7

recessive

Question 8

How can you determine whether recombination has occured?

When you have recombin between two alleles that are close, will the events be rare?

Answer 8

use several polymorphic markers that closely flank the gene

yes, because they would still be inherited together if they were really close i.e. you will still have the marker.