LIMITATIONS AND TOOLS OF THIS FIELD Flashcards
1
Q
what does off target mean?
A
That your modification etc. reaches a target you didn’t want it to.
2
Q
Why can RNAi sequence cause degradation of non target mRNAs?
A
Because it is homologous
3
Q
How much percent of transcripts can be off target when used as a trigger for PTGS?
A
50-70%
4
Q
Why can off target effects cause biosafety and environmentally issues?
A
Because it can affect entirely different organisms
5
Q
How can we avoid biosafety and environmentally issues?
A
By choosing a really specific dsRNA trigger (14 nt is long enough)
