Light Microscope

Question 1

What is the magnification of the eyepiece?

Answer 1

10x

Question 2

How do you calculate total magnification?

Answer 2

Eyepiece magnification x objective magnification

Question 3

On the objective lens, what does the 4x/0.1 mean?

Answer 3

4x = magnification 0.1 = numerical aperture

Question 4

What is refraction?

Answer 4

light bends as it slows through mediums

Question 5

How do you calculate refractive index?

Answer 5

n (refractive index) = c (speed of light in vacuum)/v (speed of light in given medium)

Question 6

What is interpupillary distance?

Answer 6

distance between eyepieces.

Question 7

How do you calculate HPD (high power objective diameter FOV)?

Answer 7

HPD = [LPD (low poer objective diameter FOV) X LPM (low power magnification)] / HPM (high power magnification)

Question 8

Why is it called compound light microscope?

Answer 8

Uses at least two lenses to magnify the object

ocular lens and the objective lens

Question 9

Memorize parts of microscope.

Answer 9