Lesson 5: Microscopy

Question 1

What is Magnification?

Answer 1

The number of times larger an image appears compared to the real size of the structure

Question 2

What is Resoltion?

Answer 2

The ability to distinguish between two separate objects

Question 3

What are the different types of Microscopes?

Answer 3

-Light Microscope
-Laser Scanning Microscope
-Transmission Electron Microscope
-Scanning Electron Microscope

Question 4

What are Light Microscopes?

Answer 4

-Use beams of light to produce and image
-Light passes through specimen
-Two magnifications (Objective Lens and Eyepiece Lens)
-Maximum magnification: x1500-x2000
-Resolution: 200nm

Question 5

What are Laser Scanning Microscopes?

Answer 5

-Use laser lights to scan an object point by point
-Depth Selectivity
-Used to observe whole living specimens

Question 6

How are Electron Microscopes different?

Answer 6

-Use a beam of electrons
-Focused using magnets
-Dehydrated specimens

Question 7

What are Transmission Electron Microscopes?

Answer 7

-Electrons pass through the specimen
-2D greyscale images
-Maximum magnification: x2000000
-Resolution: 0.5nm

Question 8

What are Scanning Electron Microscopes?

Answer 8

-Electrons reflect off of the specimen
-3D greyscale images
-Maximum magnification: x200000
-Resolution: 3-10nm