LESSON 3: SPECIMEN HANDLING AND PROCESSING Flashcards
All steps taken before the actual testing
➢ Specimen handling, processing, and blood
collection are part of this phase. Patient
identification, preparation of materials.
Pre-analytical phase (Pre-examination)
This is the actual testing phase. All procedures belonging to measurement methods belong to this phase
Analytical phase (Examination)
Processes after the test performance such as
reporting of results, interpolation of results,
recording of results, relaying of results to
physician. Everything done after test
performance.
Post-analytical phase (Post-examination)
Factors that alter test results that are introduced into the specimen before testing, including before and during collection, and during transport, processing, and storage.
Pre-analytical errors
PRE-ANALYTICAL VARIABLES
○ Physiologic
○ Lifestyle (smoking, diet, exercise, alcohol intake)
○ Drug administration
Controllable
PRE-ANALYTICAL VARIABLES
Non-controllable includes:
Biological Influences
Environmental factors
Long term cylindrical changes
Underlying Medical conditions
high altitude could affect the
Oxygen level
Heat expands the plasma volume and could cause significant decrease on
plasma protein
Factors affecting the variation of results
Patient Preparation
Affects skeletal muscle enzymes
Exercise
If you exercised before collection, your
_____________ may be increased falsely
aldolase and creatine kinase
Basal state collection means blood is collected
early in the morning before rising
Glucose requires _______ hours of fasting
6 - 8 hrs
lipid profile (triglyceride, total cholesterol, HDL, LDL) requires ______ fasting
10-12 hours maximum of 14.
If both glucose and lipid profile is need, required fasting is __________
8-12 hrs
serotonin-rich banana and tomato increases
production of
5-HIAA, a metabolite of serotonin
Sudden position change could result to
Hemoconcentration and Hemodilution
Also known as circadian rhythm
Physiologic Variation
