Lesson 2: Bacteriology (Agar Plates and Interpretation) Flashcards
Enriched Media/Non selective Isolation Media like sheep blood agar and chocolate agar
specific nutrient, grows particular pathoge
Selective media
one or more agents that are inhibitory to all organisms except those being sought
nutrients that support growth of most non-fastidious organisms
Supportive Media/General Isolation Media
Differential Media (Ex. MacConkey Agar)
allows colonies of one bacterial species to exhibit certain metabolic or culture characteristics that can be used to distinguish them from other bacteria growing on the same agar plate
Isolates and differentiates lactose fermenting and non-lactose fermenting enteric bacilli
MacConkey Agar
Modified Thayer Martin Agar
Selective for N. gonorrhoeae and N. meningitidis and supports growth of Francisella and Brucella spp.
An agar selective for vibrio spp.
Thiosulfate-Citrate-Bile Salts-Sucrose Agar
Cultuvates fastidious microoganisms such as Haemophilus spp., Brucella spp., and pathogenic Neisseria spp.
Chocolate Agar
Cultivates non-fastidious microorganisms, determination of hemolytic reactions
BAP
Differentiates enteroB, salmonella and shigella based on their ability to produce hydrogen sulfide, spit indole from tryptophan and exhibit motility
Sulfide Indole Motility
Lysine Iron Agar
uses three parameters such as
Lysine decarboxylation
Lysine deamination
H2S producton
for identifying enteroB
differentiates glucose fermenters from non-glucose fermenters and also contains sucrose and lactose, gas production duting glucose fermentation and H2S production
Trple Sugar Iron
termed as complete hemolysis characterized by a clear zone surrounding the colonies
Beta-hemolysis
No hemolysis
Gamma-hemolysis
In MAC, lactose fermenters colonies turn _____ or ____ l, while, non-lactose fermenters do not change color
red or pink
