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Hema 2 Laboratory > Lesson 1: Blood Smear Preparation & Staining > Flashcards

Lesson 1: Blood Smear Preparation & Staining Flashcards

1
Q

purpose of a blood smear is to view cellular components and evaluate:

A

number, size, morphology, and structure

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2
Q

best specimen for blood smearing

A

capillary blood

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3
Q

disadvantages of capillary blood

A
  1. platelet clumping (direct drop or heparinized)
  2. limited amount of blood can be acquired
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4
Q

specimen for blood smearing

A

fresh, venous blood in EDTA tube

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5
Q

reason for using EDTA Tube for blood smearing:

A

prevents platelet clumping on the glass slide

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6
Q

storage time of EDTA tube:

A

within 4 hours

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7
Q

disadvantage of using EDTA tube:

A

Blood artifacts (if stored in RT for 5 hours)

  1. RBC: spherocytes, necrobiotic leukocytes, vacoulated neutrophils
  2. platelet satellitosis: PSEUDOTHROMBOCYTOPENIA
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8
Q

platelet satellitosis:

A

PSEUDOTHROMBOCYTOPENIA

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9
Q

Techniques in blood smearing

A
  1. Manual Wedge Technique
  2. Coverslip Technique (Ehrlich)
  3. Beckman Coulter
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10
Q

ideal angle smearing for manual wedge technique:

A

30-45 degree

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11
Q

blowing on slides would cause:

A

moisture accumulation

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12
Q

moisture accumulation by blowing on slides would lead to:

A

ECHINOCYTIC or crenated RBC

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13
Q

features of a well-made wedge peripheral blood film:

A
  1. film is 2/3 of 3/4 length of the glass slide
  2. should have a finger-shaped smear
  3. lateral edges should be visible
  4. should be smooth without irregularities, holes, and streaks
  5. when held up to the light, rainbow-appearance should be visible
  6. whole blood is placed and smeared.
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14
Q

factors of a thick blood smear

A

a. too large of blood drop
b. too fast
c. too high angle of speader

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15
Q

factor of a thin blood smear

A

a. too small/thin drop of blood
b. too slow
c. too low angle of spreader

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16
Q

rare method of peripheral blood films

A

coverslip technique (ehrlich)

17
Q

coverslip technique is used for:

A

bone marrow aspirated smear

18
Q

advanced technique for blood smearing

A

beckman coulter

19
Q

automated slide making & staining system

A

beckman coulter

Hema 2 Laboratory (1 decks)

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