Lesson 1 Flashcards
List the 5 components of comprehensive lab safety program
1) Procedures & Precautions for use & maintenance of equipment.
2) Safety equipment & supplies: eye wash station, fire extinguishers, spill clean up kit, hazardous & biohazard waste containers & protective gloves
3) Training of all employees on location and usage of all these items
4) Lab safety policies must be in writing & placed in accessible location in lab.
5) Sign must me posted that says “no eating, drinking, applying cosmetics or adjusting contacts in lab”
List the parts of the microscope.
Parts of the microscope include eye pieces (ocular lenses), the nose piece, the objective lenses, the stage, the slide holder, travel knobs, which are also referred to as mechanical stage control knobs, fine adjustment knob, course adjustment knob, condenser, diaphragm, light source.
Function of the eye piece (ocular lens)
Ocular lenses are the first points of magnification
Function of the Objective Lens
Objective lenses is the second point of magnification. Most compound microscopes have three to four, although you can have compound microscopes with more than this. 4x is scanning power, usually a red color. 10x is the low power objective, typically yellow. 40x is high and dry objective, so high power, but it’s dry because we do not use oil on this objective, usually a blue color. And then the 100x which is our oil immersion, that is a white or gray color.
Function of the stage/slide holders
The stage is where we put our microscope slide. There’s a little arm that helps hold the slide in place.
Function of the travel knobs, or mechanical stage control knobs
Helps to move our slide back and forth so we can use it to scan.
Function of the fine adjustment knob
The fine adjustment knob moves the stage ever so slightly that you will not be able to see it with your naked eye.
Function of the course adjustment knob
The coarse adjustment knob is the one that moves the stage the largest.
Function of the condenser
Your condenser sits just below the stage. It is sometimes referred to as the substage condenser. It has two lenses that will focus the light from the light source below up onto the specimen.
Function of diaphragm
You use the diaphragm to control the amount of light that is directed up at the slide. More light will typically clarify the specimen.
Function of the light source
The light source is found in your base of your microscope. Your light source is typically on a dimmer like switch so you can control the intensity of the light.
Discuss the use, care, and maintenance of the microscope.
- Only high-quality lens tissue should be used to clean the lenses.
- The microscope should be wiped clean after each use and kept covered when not in use.
- The eyepiece is cleaned with lens paper.
- Cleaning and adjustment by a microscope professional should be performed at least annually.
Describe methods for the use, care and maintenance of clinical centrifuges.
- Ensure the load is balanced and tightly sealed
- Do not open until spinning has completely stopped.
- Refer to maintenance schedule in operator’s manual
- Bearing may need to be lubricated and cleaned periodically
- Periodic testing of speed and time is good practice to ensure proper test results.
Describe methods for the use, care and maintenance of refractometers
- Inspect and clean the prism cover glass and cover plate.
- Place a drop of sample fluid on the prism cover glass, and close the cover.
- Point the refractometer toward bright artificial light or sunlight.
- Bring the light-dark interface line into focus by turning the eyepiece.
- Read and record the result with the appropriate scale (e.g., specific gravity, protein).
- Clean the refractometer according to the manufacturer’s recommendations.
