Lesson 1 Flashcards
the study of the chemical substances and processes that occur in plants, animals, and microorganisms and of the changes they undergo during development and life.
BIOCHEMISTRY
It deals with the chemistry of life, and as such it draws on the techniques of analytical, organic, and physical chemistry, as well as those of physiologists concerned with the molecular basis of vital processes.
BIOCHEMISTRY
All chemical changes within the organism—either the degradation of substances, generally to gain necessary energy, or the buildup of complex molecules necessary for life processes.
METABOLISM
These chemical changes depend on the action of organic catalysts known as
ENZYMES
The ultimate goal of biochemistry
EXPLAIN ALL LIFE PROCESSES IN MOLECULAR DETAIL
the study of the chemical substances and vital processes occurring in living organisms; Biological Chemistry; Physiological Chemistry.
BIOCHEMICAL SYSTEM
Processes occurring under Normal Conditions
PHYSIOLOGICAL
Those arising under Abnormal Conditions are ____. They fall under CLINICAL BIOCHEMISTRY.
PATHOLOGICAL
employ the techniques and theories of chemistry to probe the molecular basis of life.
they have established, among other things, the principles that underlie energy transfer in cells, the chemical structure of cell membranes, the coding and transmission of hereditary information, muscular and nerve function, and biosynthetic pathways.
BIOCHEMISTS
the study of compounds of carbon and hydrogen and their derivatives. Because the cellular apparatus of living organisms is made up of carbon compounds, biomolecules are part of the subject matter of organic chemistry.
ORGANIC CHEMISTRY
determined by comparisons of ribosomal RNA (rRNA) sequences that it is possible to construct a highly accurate tree of life showing the evolutionary relationship between all life forms.
CARL WOESE
One of the most important enzymes used in modern biochemistry laboratories
TAQ POLYMERASE
Taq polymerase enzyme is derived from the Archaean species ___
Thermus Aquaticus
the lowest level of structure capable of performing ALL the activities of life.
CELL
The first cells were observed and named by_____ in _____ from the slice of cork.
ROBERT HOOKE ; 1665
who proposed the cell theory
MATTHIAS SCHLEIDEN and THEODOR SCHWANN in 1839
Some organisms consist of a single cells = unicellular organisms, called
PROKARYOTES
multicellular aggregates of specialized cells
EUKARYOTES
Types of Cell
Bacteria
Archaea
Protista
Fungi
Plantae
Animalia
size of cells which can be visualized by a light microscope
1 to 100 um in diameter
Types of Microscopes
Light Microscope
Electron Microscope
visible light is passed through the specimen and then through glass lenses
Light Microscope
the ratio of an object to its real size
Magnification
Magnification of Light Microscope
x1000
The measure of image clarity
The minimum distance two points can be separated and still viewed as two separate points
Resolving Power
Limited by the shortest wavelength of light- one-half of the wavelength used
Resolution
The minimum resolution of Light Microscope
About 2 microns, the size of a small bacteria
It focuses a beam of electrons through specimen or onto its surface
Electron Microscope
Resolution of modern Electron Microscope
0.1 nm but practical limit is about 2 mm
Study the internal ultrastructure of cells ; 2D
Electron beam was aimed through the thin section of specimen
Transmission Electron Microscope
Study the surface structure of the cell; 3D
Sample surface is covered with the thin film of gold
Scanning Electron Microscope
to separate the cells for functional study
Cell Fractionation
The disrupted cells are centrifugated at different speed and duration to fractionate components of different species
Cell Fractionation
It prepares the quantities of specific cell components/organelles for functional analysis, referred to as BIOCHEMICAL STUDIES
Cell Fractionation
The major tool in cytology
Microscope
The study of molecules and chemical processes in metabolism, developed Modern Cell Biology
Cytology
Are bounded by Plasma Membrane/Cell Membrane/Plasmalemma
Cells
Semifluid substance within the membrane that contains the organelles
Cytosol