Lesson 1 Flashcards
DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome.
Recombinant
rDNA molecules are sometimes called
chimeric DNA
the simplest form of genome editing that can be achieved using programmable nucleases
gene disruption
in this technique, a gene gun is used to fire DNA coated pellets on plants
biolistic
a process used to transfer plasmid DNA into badteria
plasmid insertion by heat shock treatment
In the laboratory, ________ (or restriction endonucleases) are used to cut DNA into smaller fragments.
restriction enzymes
join together
ligation
is a process used to transfer plasmid DNA into bacteria
heat shock treatment
This technique follows a similar methodology as Heat shock
Treatment, but, the expansion of the membrane pores is done through an electric “shock” This method is commonly used for insertion of genes into mammalian cells.
electroporation
a selection marker within the inserted plasmid DNA sequence allows the selection of “transformants”
selection of plasmid DNA containing cells
certain inserted genes within the plasmids provide visible proof of their presence
selection of transformed cells with the desire gene
alternatively, the presence of the desired genes in the inserted plasmids may be confirming using PCR amplification
PCR detection of plasmid DNA
with the ability to insert gene sequences, comes the possibility of providing new traits for these target organisms
genetically modified organisms (GMOs)
tomato was the first generically modified organism that was licensed for human consumption
the flavr savr
was the first developed to incorporate the production of toxic (i.e b-endotoxin) from Bacillus Thuringensis in corn plants.
