Lesson 1 Flashcards
block containing tissue is placed in an instrument called
Microtome
Important embedding medium for the clearing stage
Toluene
Basophilic
Cell components such as nucleic acids with a
net negative charge (anionic) stain more
readily with basic dye
Cationic
cationic components, such as proteins with
many ionized amino groups, have affinity for
acidic dyes
Basic dyes
Toluidine blue
Alcian blue
Methylene blue
Hematoxilin
Behaves like basic dye staining basophilic tissue components
The main tissue components that ionize and react with basic dyes
do so because of acids
in their composition (DNA, RNA, and glycosaminoglycans)
Acid dyes
Eosin
Orange G
Acid fuchsin
Stain the acidophilic components of tissues mitochondria, secretory granules, and collagen.
H&E
Hematoxylin dark blue or purple staining the dna in the cell neucleus and other acidic structures like Rna rich portions of cytoplasm and the matrix of cartilage
Eosin stains cytoplasmic components and collagen pink
Chromophobic
Clear cell
Amphiphilic
Basophilic and eosinophilic
Periodic acid-schiff (PAS)
carbohydrates and carbohydrate rich molecules.
Used for:
- glycogen in cells
- mucus in various cells and tissues
- the basement membrane that underlies epithelia
- reticular fibers in connective tissue
H&E stained
Nuclei stained purple
Cytoplasm stains pink oligosaccharides and glycoproteins like the ends of the cells at lumen or the scattered mucus secreting goblet cells are poorly stained
PAS
cell staining is most intense at the lumen,
where projecting microvilli have a prominent layer of glycoproteins at the lumen (L) and in
the mucin-rich secretory granules of goblet cells.
Masson’s trichrome
a three-color staining protocol used for distinguishing cells
from surrounding connective tissue.
Masson’s trichrome
red keratin and muscle fibers, blue or green collagen and bone,
light red or pink cytoplasm, and dark brown to black cell nuclei.
