Lescture 1 Flashcards
What is somato sensation?
Gathering sensory a information from the eyes ears nose skin est
How does sensation reach brain?
Ap from the sensory organelle to specific regains of the carebal contextalong somato sensory neurones.
What cell and molecule transducer the sense of olfaction
The all is the olfactory vorone I which senses using gpcrs(chenosensation)
What cell and molecule transdu as vision?
Photo-ceptarcelland the gpcr rhodopsin
What call and molecule transducerhearing?
Inner hair cell (mechanocensathechannel)
What cell , New one and molecule transducer touch
A beta neurone + non neurone cell along with the piezo 2 channel
What neuron and molecule transducer the sense of poin
A delta or c-narone with a trp channel
What New one and molecule transducer the sense of itch ( chemical)
C- neurone with gpcr and trp channels
Why is sa mouse a good test platform for identified ding somatosensory transduction?
Genetic modification is most easily performed in a mouse compared to other mammals
What are the problems of ISOla ted sensory New ones inculture)
They lack connectivity to rest of the nervous system and the natural neurone anatomy
What is the problem with ex-vivo tissue in studying somato sensation?
It retains natural new anatomy but No competivityto rest of nervous system
What are or three experimental methods macan use to decinefer somotosenation
Isolated sensors neurones inculture, ex-vivo tissue, living conscious mice
What is the difference between knock toward knock out thechigues
Knock down →→ ssirna→ reduces the expression ofa particular protein
Knock out → Gene deleted fora particular protein, total ko is from all cells where as conditional to is from only sono alls
How do we perform cell specific knock out
Cell type specificgene promotors canbe exploited to low for knockout, transgene expression
Now to prepare isolated sensory New ones
Spine dissected → dorsal rootgengia picked → extra cellular matrix digested with collongerase to seperatureneronos→Culture
What a calcium sensor used in imaging ?
Fura 2 is Ca 2+ sensitive dye which increases sin Florence when bound to ca2+
What is insitue hybridisation?
The detection of specific mRNAs
What is immune his to chemistry?
Detection of specific proteins’
What stain dove use to stain brain sections what does it allows to differiate ?
Cresyl violet stain → binds to nevoral nuclei and rough endoplasmic reticulum → so allows for difficuliated grey matter from white matter (grejis darker)
What is a Von Frey filament, what does it test for
Thin filaments of nylon which buckle when the right force is applied used to test nocioception1 increase of filament thickness increases force transducer allowing for the determination of minimum force needed to produce a response
