Lecture KH7. Purification, detection and characterization of proteins 2 Flashcards
What is the ultimate breakthrough that GFP (green fluorescent protein) brought about? (mentioned briefly in the lecture)
For the first time, there is no need to kill the animal in order to look at it in the living cells
It has also played a role in viewing the distribution of proteins in the living
What is GFP ( green fluorescent protein) and how can it be used?
It is a jellyfish protein that fluoresces green
It is a single polypeptide chain that contains enzymatic activity that modifies some of its own amino acids side chains to generate the fluorochrome
it can be used as a reporter gene for transcriptional control elements
it can be made into fusion proteins to study intracellular protein localization
(it has also been transformed into different colours! )
Where does the energy for polymerization come from?
It comes from the breakage of either the alpha or beta phosphate (from dNTP)
The phosphate leaves to form a bond with the 3’ hydroxyl group (OH)
